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Purification of L-Lysine in Simulated Moving Bed and Fixed-Bed Chromatography

ROBATJAZI Seyed Mortaza1, SHOJAOSADATI Seyed Abbas1, KARBASY Seyed Mojtaba2   

  1. 1. Biotechnology Group, Department of Chemical Engineering, Tarbiat Modarres University,Tehran, Iran; 2. Institute of Jahad Engineering, Tehran, Iran
  • 收稿日期:2003-11-27 修回日期:2004-02-12 出版日期:2004-07-30 发布日期:1989-12-25
  • 通讯作者: SHOJAOSADATI Seyed Abbas
  • 基金资助:

Purification of L-Lysine in Simulated Moving Bed and Fixed-Bed Chromatography

OBATJAZI Seyed Mortaza1, SHOJAOSADATI Seyed Abbas1, KARBASY Seyed Mojtaba2   

  1. 1. Biotechnology Group, Department of Chemical Engineering, Tarbiat Modarres University,Tehran, Iran; 2. Institute of Jahad Engineering, Tehran, Iran
  • Received:2003-11-27 Revised:2004-02-12 Online:2004-07-30 Published:1989-12-25
  • Contact: SHOJAOSADATI Seyed Abbas

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摘要： L-Lysine was produced by a microbial process utilizing a Corynebacterium glutamicum (ATCC 21799) strain. L-Lysine was purified from the cultivated medium by fixed-bed and simulated moving bed （SMB） chromatography. The separation conditions including pH, eluent concentration and Lys+ and Lys2+ adsorption isotherms were studied in batch adsorption. The column capacity, eluent flow rate and eluent concentration have been studied in fixed-bed chromatography. Maximum purification rate of lysine was obtained as 0.066 g/(g·h) (per gram resin and per hour) at an eluent flow rate of 10 mL/min in fixed-bed chromatography. The results obtained from SMB were 0.11 g/(g·h) for L-lysine purification rate and 96% for L-lysine recovery.

关键词: L-lysine , purification, simulated moving bed, ion exchange chromatography

Abstract: L-Lysine was produced by a microbial process utilizing a Corynebacterium glutamicum (ATCC 21799) strain. L-Lysine was purified from the cultivated medium by fixed-bed and simulated moving bed （SMB） chromatography. The separation conditions including pH, eluent concentration and Lys+ and Lys2+ adsorption isotherms were studied in batch adsorption. The column capacity, eluent flow rate and eluent concentration have been studied in fixed-bed chromatography. Maximum purification rate of lysine was obtained as 0.066 g/(g·h) (per gram resin and per hour) at an eluent flow rate of 10 mL/min in fixed-bed chromatography. The results obtained from SMB were 0.11 g/(g·h) for L-lysine purification rate and 96% for L-lysine recovery.

Key words: ion exchange chromatography； simulated moving bed； purification； L-lysine