色谱

色谱

• 技术与应用 • 上一篇    下一篇

固相萃取-高效液相色谱法测定啤酒中的黄腐酚

王宁1,2, 李永仙1,2, 郑飞云1,2, 刘春凤1,2, 李崎1,2*, 顾国贤2   

  1. 1.Jiangnan University, Key Laboratory of Industrial Biotechnology of Ministry of Education, Wuxi 214122, China; 2.Lab of Brewing Science and Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, China
  • 收稿日期:2008-10-09 修回日期:2008-12-19 出版日期:2009-05-30 发布日期:1982-09-25
  • 通讯作者: 李崎

Determination of xanthohumol in beer by solid-phase extraction-high performance liquid chromatography

WANG Ning1,2, LI Yongxian1,2, ZHENG Feiyun1,2, LIU Chunfeng1,2, LI Qi1,2*, GU Guoxian2   

  1. 1.Jiangnan University, Key Laboratory of Industrial Biotechnology of Ministry of Education, Wuxi 214122, China; 2.Lab of Brewing Science and Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, China
  • Received:2008-10-09 Revised:2008-12-19 Online:2009-05-30 Published:1982-09-25
  • Contact: LI Qi

PDF

689

被引次数

15

摘要： 采用Waters Sep-Pak C18固相萃取小柱对啤酒样品进行分离纯化,建立了啤酒中黄腐酚的固相萃取-高效液相色谱检测方法。选用色谱柱Zorbax Eclipse XDB-C18柱(250 mm×4.6 mm,5 μm),以甲醇和0.1%甲酸水溶液为流动相进行梯度洗脱,柱温25 ℃,流速0.4 mL/min,检测波长370 nm。在此条件下,黄腐酚分离良好且无杂质峰干扰,在0.5～500 μg/L的范围内线性关系良好(r21),在高、中、低浓度下的加标回收率为91.21%～95.58%,相对标准偏差小于2%。方法的检出限为0.24 μg/L,定量限为0.80 μg/L。该方法简便快速、结果准确、重现性好,是检测啤酒中黄腐酚含量的有效方法。

关键词: 高效液相色谱法, 固相萃取, 黄腐酚, 啤酒

Abstract: A method for the determination of xanthohumol in beer using solid-phase extraction-high performance liquid chromatography (SPE-HPLC) has been developed. A Waters Sep-Pak C18 column was used to extract and clean-up the sample. The separation was achieved on a reversed-phase Agilent Zorbax Eclipse XDB-C18 (250 mm×4.6 mm, 5 μm) in a linear gradient, and the mobile phases were consisted of water (containing 0.1% formic acid) (A) and methanol (B) with a flow rate of 0.4 mL/min. In addition, the column temperature was maintained at 25 ℃. The detection wavelength was set at 370 nm. There was a good linear relationship (r21) in the range of 0.5~500 μg/L. The average recoveries were between 91.21% and 95.58% with the relative standard deviations less than 2%. The limit of detection was 0.24 μg/L and the limit of quantification was 0.80 μg/L. It was proved to be a convenient and accurate method for the analysis of xanthohumol content in beer.

Key words: beer , solid-phase extraction (SPE), xanthohumol, high performance liquid chromatography (HPLC)