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色谱

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聚十二烷基甲基丙烯酸酯整体柱的制备及其在毛细管电色谱法分离肌红蛋白酶解产物中的应用

王婷婷1,2, 梁振1, 张丽华1*, 张玉奎1   

  1. 1. 中国科学院大连化学物理研究所, 中国科学院分离分析化学重点实验室, 国家色谱研究分析中心, 辽宁 大连 116023; 2. 中国科学院研究生院, 北京 100049
  • 收稿日期:2009-12-09 修回日期:2010-01-09 出版日期:2010-03-28 发布日期:1981-03-25
  • 通讯作者: 张丽华

Preparation of monolithic polylaurylmethacrylate column and its application in capillary electrochromatographic separation of myoglobin digests

WANG Tingting1,2, LIANG Zhen1, ZHANG Lihua1*, ZHANG Yukui1   

  1. 1. National Chromatographic R. & A. Center, Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; 2. Graduate School of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2009-12-09 Revised:2010-01-09 Online:2010-03-28 Published:1981-03-25
  • Contact: ZHANG Lihua

PDF

642

被引次数

7

摘要： 以十二烷基甲基丙烯酸酯(LMA)为功能单体,乙叉二甲基丙烯酸酯(EDMA)为交联剂,正丙醇、1,4-丁二醇和水为三元致孔剂,以及2-丙烯酰胺-2-甲基丙磺酸(AMPS)为电渗流产生剂,制备了聚十二烷基甲基丙烯酸酯整体柱。系统考察了AMPS含量和单体-致孔剂比例对柱性能的影响。结果表明,单体溶液和致孔剂的最佳聚合溶液质量比为35:65,其中单体溶液组成为59.5%（质量分数,下同）LMA、40%EDMA和0.5%AMPS,致孔剂溶液组成为60%正丙醇、30%1,4-丁二醇和10%水。在优化的流动相条件下应用制备的整体柱采用毛细管电色谱法成功地分离了肌红蛋白酶解产物。

关键词: 肌红蛋白酶解产物, 毛细管电色谱法, 十二烷基甲基丙烯酸酯, 整体柱

Abstract: The monolithic polylaurylmethacrylate column was prepared in a single step using the monomer solution containing lauryl methacrylate (LMA), ethylene dimethacrylate (EDMA), and 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS), with the ternary porogenic solvent consisting of 1-propanol, 1,4-butanediol and H2O. The effects of AMPS content and the ratio of monomer solution to porogenic solvent were investigated. The optimal mass ratio of monomer solution to porogenic solvent was 35:65, the monomer solution was composed of 59.5%（w/w） LMA, 40%（w/w） EDMA and 0.5%（w/w） AMPS, and the porogenic solution was composed of 60%（w/w） 1-propanol, 30%（w/w） 1,4-butanediol and 10%（w/w） H2O. The prepared monolithic column was successfully applied in the capillary electrochromatographic (CEC) separation of myoglobin digests under the optimized mobile phase.

Key words: lauryl methacrylate, monolithic column , myoglobin digests, capillary electrochromatography （CEC）