色谱

色谱

• 研究论文 • 上一篇    下一篇

高效液相色谱-质谱法测定发酵液中的喷司他丁

杨鹏*, 王岩, 廖艳艳   

  1. 上海医药工业研究院, 上海 200040
  • 收稿日期:2009-08-07 修回日期:2009-10-07 出版日期:2010-03-28 发布日期:1981-03-25
  • 通讯作者: 杨鹏

Determination of pentostatin in culture broth using high performance liquid chromatography-mass spectrometry

YANG Peng*, WANG Yan, LIAO Yanyan   

  1. Shanghai Institute of Pharmaceutical Industry, Shanghai 200040, China
  • Received:2009-08-07 Revised:2009-10-07 Online:2010-03-28 Published:1981-03-25
  • Contact: YANG Peng

PDF

659

被引次数

11

摘要： 建立了测定发酵液中喷司他丁含量的反相高效液相色谱-质谱分析方法。采用的色谱条件: 色谱柱为Hypersil ODS2柱(250 mm×4.6 mm, 5 μm);流动相为甲醇/乙腈/10 mmol/L乙酸铵(pH 7.6)(2.5/2.5/95, v/v/v),流速为1.0 mL/min;检测波长为280 nm;柱温为40 ℃;进样量为10 μL。喷司他丁在1.0～100 mg/L范围内有良好的线性关系,相关系数为0.9999。该方法精密度好,稳定性高,能简便、快速、准确地测定发酵液中喷司他丁的含量。

关键词: 发酵液 , 高效液相色谱-质谱法, 喷司他丁

Abstract: A method for the determination of pentostatin in culture broth was established using high performance liquid chromatography-mass spectrometry (HPLC-MS). The chromatographic conditions used were as follows: the analytical column was Hypersil ODS2 (250 mm×4.6 mm, 5 μm); the mobile phase was methanol/acetonitrile/10 mmol/L ammonium acetate (pH 7.6) (2.5/2.5/95, v/v/v) with a flow rate of 1.0 mL/min; the detection wavelength was 280 nm; the injection volume was 10 μL; the temperature was 40 ℃. The linear range of the method for pentostatin was 1.0－100 mg/L (r=0.9999). The method is characterized by simplicity, higher sensitivity and accuracy.

Key words: culture broth , pentostatin, high performance liquid chromatography-mass spectrometry (HPLC-MS)