色谱

色谱 ›› 2011, Vol. 29 ›› Issue (03): 277-280.DOI: 10.3724/SP.J.1123.2011.00277

• 技术与应用 • 上一篇    

低速逆流色谱分离制备栀子黄色素中的藏花素

蒋志国1*, 陈文学1, 刘四新1, 杜琪珍2   

  1. 1. 海南大学食品学院, 海南 海口 570228; 2. 浙江工商大学食品与生物工程学院, 浙江 杭州 310012
  • 收稿日期:2010-11-14 修回日期:2011-01-05 出版日期:2011-03-28 发布日期:2011-03-28
  • 通讯作者: 蒋志国,讲师.

Preparation of crocin from gardenia yellow pigment by slow rotary countercurrent chromatography

JIANG Zhiguo1*, CHEN Wenxue1, LIU Sixin1, DU Qizhen2   

  1. 1. College of Food Science, Hainan University, Haikou 570228, China; 2. College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310012, China
  • Received:2010-11-14 Revised:2011-01-05 Online:2011-03-28 Published:2011-03-28

PDF

639

被引次数

26

摘要： 建立了低速逆流色谱技术(SRCCC)从栀子黄色素中快速分离制备藏花素的方法。两相溶剂系统由叔丁基甲基醚-正丁醇-乙腈-水(2:2.5:1:5, v/v/v/v)组成,以上相为固定相,下相为流动相。在转速为50 r/min和流速为5 mL/min的条件下,从5 g栀子黄色素粗样中分离得到2.47 g藏花素,纯度为96.8%。该方法制备量大、安全、高效,有可能成为工业级分离制备藏花素的一种有效手段。

关键词: 藏花素, 低速逆流色谱, 栀子黄色素, 制备

Abstract: A method was established for the preparation of crocin from gardenia yellow pigment by slow rotary countercurrent chromatography (SRCCC). A two-phase solvent system consisting of methyl tert-butyl ether, n-butanol, acetonitrile and water (2:2.5:1:5, v/v/v/v) was used. The upper phase was used as the stationary phase, and the lower phase as the mobile phase. By SRCCC elution, 2.47 g of crocin with a purity of 96.8% was obtained from 5 g of gardenia yellow pigment at a flow rate of 5 mL/min and a rotation speed of 50 r/min. The results indicated that SRCCC is a powerful technique for the purification of crocin from gardenia yellow pigment with high preparative capacity, high safety and high efficiency in resolution, and it is possible to prepare crocin at industrial level by SRCCC.

Key words: crocin, gardenia yellow pigment, preparation, slow rotary countercurrent chromatography