色谱

色谱 ›› 2011, Vol. 29 ›› Issue (02): 162-167.DOI: 10.3724/SP.J.1123.2011.00162

• 研究论文 • 上一篇    下一篇

固相萃取-高效液相色谱法同时测定调味品中15种工业合成染料

刘敏1,2, 李小林2*, 别玮2, 王明林1*, 冯骞2   

  1. 1. 山东农业大学食品科学与工程学院, 山东 泰安 271000; 2. 北京出入境检验检疫局, 北京 100026
  • 收稿日期:2010-10-26 修回日期:2010-12-23 出版日期:2011-02-28 发布日期:2007-03-25
  • 通讯作者: 李小林,硕士,工程师. Tel: (010)58619225.王明林,博士,硕士生导师.
  • 基金资助:

    北京市科委重大科技计划项目(No. D08050200310801)

Simultaneous determination of 15 industrial synthetic dyes in condiment by solid phase extraction-high performance liquid chromatography

LIU Min1,2, LI Xiaolin2*, BIE Wei2, WANG Minglin1*, FENG Qian2   

  1. 1. College of Food Science and Engineering, Shandong Agricultural University, Taian 271000, China; 2. Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China
  • Received:2010-10-26 Revised:2010-12-23 Online:2011-02-28 Published:2007-03-25

PDF

958

被引次数

75

摘要： 建立了测定调味品中15种工业合成染料的固相萃取-高效液相色谱法(SPE-HPLC)。样品经甲醇-水(1:1, v/v)超声提取、SPE柱净化后用HPLC进行分析,流动相为10 mmol/L乙酸铵溶液(含1%乙酸)和乙腈。实验结果表明15种工业合成染料的分离效果良好,回收率为84.6%～114.2%,相对标准偏差为0.9%～10.3%;检出限为0.05～0.18 mg/kg。该方法操作简单,结果准确,重现性好,可用于同时测定调味品中非法添加的15种工业合成染料。

关键词: 调味品, 高效液相色谱, 工业合成染料, 固相萃取

Abstract: A new method was established for the determination of 15 industrial synthetic dyes in condiment by solid phase extraction-high performance liquid chromatography(SPE-HPLC). The samples were extracted by methanol-water (1:1, v/v) and purified by a solid phase extraction column. Then, the chromatographic separation was achieved on a Luna C18 column by linear gradient elution. The mobile phase was 10 mmol/L ammonium acetate-acetonitrile (containing 1% acetic acid). The results showed that the 15 industrial synthetic dyes can be separated efficiently. The recoveries of the 15 industrial synthetic dyes spiked in condiment were between 84.6% and 114.2% with the relative standard deviations of 0.9%~10.3%. The limits of detection of this method was 0.05~0.18 mg/kg for the 15 industrial synthetic dyes. The method is simple, sensitive, accurate, repeatable and can be used for simultaneous determination of the 15 illegally added industrial synthetic dyes.

Key words: condiment, high performance liquid chromatography (HPLC), industrial synthetic dyes, solid phase extraction (SPE)

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