色谱

色谱 ›› 2011, Vol. 29 ›› Issue (03): 199-204.DOI: 10.3724/SP.J.1123.2011.00199

• 专论与综述 • 上一篇    下一篇

牛胰腺中活性蛋白质的液相色谱分离纯化方法进展

杨晓明1,2, 耿信笃2,3*   

  1. 1. 平凉医学高等专科学校, 甘肃 平凉 744000; 2. 西北大学合成与天然功能分子化学教育部重点实验室, 陕西 西安 710069; 3. 西北大学现代分离科学研究所, 陕西 西安 710069
  • 收稿日期:2010-12-03 修回日期:2011-02-10 出版日期:2011-03-28 发布日期:2011-03-28
  • 通讯作者: 耿信笃,教授,博士生导师,主要研究生物大分子的色谱分离. E-mail: xdgeng@nwu.edu.cn
  • 基金资助:
    西北大学合成与天然功能分子化学教育部重点实验室开放基金资助项目(KF09012).

Recent development for purification of active proteins from bovine pancreas with liquid chromatography

YANG Xiaoming1,2, GENG Xindu2,3*   

  1. 1. Pingliang Medicine College, Pingliang 744000, China; 2. Ministry of Education Key Laboratory of Synthetic and Natural Functional Molecular Chemistry, Northwest University, Xi’an 710069, China; 3. Institute of Modern Separation Science, Northwest University, Xi’an 710069, China
  • Received:2010-12-03 Revised:2011-02-10 Online:2011-03-28 Published:2011-03-28

PDF

992

被引次数

7

摘要： 牛胰腺中含有多种活性蛋白,其中许多蛋白已被开发为有利于人类健康的药物。从牛胰腺中分离纯化得到的蛋白药物是一种有高附加值的高技术产品。现代生物技术中所用的大多数有价值的活性蛋白产品的制备仍然依赖于不同的液相色谱法。本文综述了牛胰腺中活性蛋白质的提取方法以及以色谱分离为主的分离与纯化技术,为开展从天然产品中提取并应用蛋白质提供一定的参考。

关键词: 蛋白药物, 分离与纯化, 牛胰腺, 色谱, 制备色谱

Abstract: Many active proteins exist in bovine pancreas and some of them have become protein drugs for human heath. These protein drugs sourcing from bovine pancreas are also high-tech product having high economic benefit. In the modern biological technology, the preparation of most active protein products relies on various liquid chromatographic techniques. The recent development of extraction of the active proteins from bovine pancreas and their separations and purifications, mainly with chromatographic methods are reviewed in this paper. It would be expected to be helpful for the preparation and application of the active proteins from natural products.

Key words: bovine pancreas, preparative chromatography, protein drug, separation and purification, chromatography