色谱

色谱

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毛细管电泳在线聚焦原理、技术及应用

刘胜权, 汪海林*   

  1. 中国科学院生态环境研究中心, 环境化学与生态毒理学国家重点实验室, 北京 100085
  • 收稿日期:2011-08-22 修回日期:2011-09-03 出版日期:2011-09-28 发布日期:2011-10-28
  • 通讯作者: 汪海林,研究员,博士,博士生导师,研究方向主要包括高灵敏生物分离分析、生物分子相互作用研究和DNA损伤的检测及修复机制研究. Tel: (010)62849600
  • 基金资助:

    国家“973计划”支持项目(No. 2007CB407305)和国家自然科学基金项目(Nos. 20737003, 20877091)

Principle, technique and applications of on-line focusing in capillary electrophoresis

LIU Shengquan, WANG Hailin*   

  1. State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
  • Received:2011-08-22 Revised:2011-09-03 Online:2011-09-28 Published:2011-10-28

PDF

661

被引次数

32

摘要： 毛细管电泳以其分析速度快、分离效率高、操作简便、能够实现高通量而获得了广泛的应用,但由于检测窗口小而导致其检测灵敏度低。为了提高检测灵敏度,目前已发展了多种毛细管电泳在线聚焦和样品预浓缩技术,如场放大样品堆积、pH调节浓缩、胶束电动毛细管色谱、等速电泳等。这些技术由于能够在毛细管内同时实现样品的聚焦和分离、操作简便而获得了广泛的兴趣和关注。本文针对毛细管电泳的在线聚焦的原理、技术和应用做一简要的介绍和总结。

关键词: 堆积, 毛细管电泳, 预浓缩, 在线聚焦, 综述

Abstract: Capillary electrophoresis (CE) has wide applications in many fields because of its extraordinary advantages, such as high efficiency of separation, high speed, and high throughput. However, the detection sensitivity is relatively low due to short detection window and short optical length. To improve the detection sensitivity, a number of on-line sample focusing and stacking modes have been developed, such as field-amplified sample stacking, pH gradient preconcentration, micellar electrokinetic capillary chromatography (MEKC), isotachophoresis (ITP). These on-line focusing techniques have been of intensive interest because of the ease of operation and simultaneous focusing during capillary electrophoresis separation. Here, we review the principle, advanced techniques and latest applications of on-line capillary electrophoresis focusing.

Key words: on-line focusing, preconcentration, review, stacking, capillary electrophoresis （CE）