色谱

色谱 ›› 2011, Vol. 29 ›› Issue (12): 1240-1243.DOI: 10.3724/SP.J.1123.2011.01240

• 技术与应用 • 上一篇    下一篇

高效液相色谱法测定食品中氟啶脲的残留量

杜利君*, 宋洁, 张丽娜, 刘红艳   

  1. 山西出入境检验检疫局技术中心, 山西 太原 030024
  • 收稿日期:2011-08-04 修回日期:2011-10-05 出版日期:2011-12-28 发布日期:2012-01-15
  • 通讯作者: 杜利君,工程师,主要从事农药残留、兽药残留、食品添加剂检测等. Tel: (0351)6160663

Determination of chlorfluazuron residue in foods by high performance liquid chromatography

DU Lijun*, SONG Jie, ZHANG Lina, LIU Hongyan   

  1. Technology Center of Shanxi Entry-Exit Inspection and Quarantine Bureau, Taiyuan 030024, China
  • Received:2011-08-04 Revised:2011-10-05 Online:2011-12-28 Published:2012-01-15

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摘要： 建立了高效液相色谱测定食品中氟啶脲残留量的方法。样品中的氟啶脲经正己烷或乙腈提取,弗罗里硅土净化后,以乙腈-水(85:15, v/v)混合溶液为流动相,经C18色谱柱分离,紫外检测器(260 nm)测定。结果表明: 氟啶脲在0.05～2.0 mg/L范围内线性良好(相关系数为0.9998),定量限(以信噪比为10计)为0.05 mg/kg。在0.05、0.1、1 mg/kg添加水平下的加标回收率为82.1%～102.5%,相对标准偏差(n=10)为3.00%～6.25%。该方法操作简便、快速,稳定性好,样品和溶液用量少,可用于水果、蔬菜、粮谷、动物源性等食品中氟啶脲残留量的日常检测。

关键词: 氟啶脲, 高效液相色谱法, 食品

Abstract: A method for the determination of chlorfluazuron residue in foods by high performance liquid chromatography (HPLC) was developed. The samples were extracted with hexane or acetonitrile. After cleaned up with Florisil-solid phase extraction (SPE), the samples were analyzed by the separation on a C18 chromatographic column with an acetonitrile and water (85:15, v/v) mixed solution as the mobile phase and the determination with an ultraviolet detector at 260 nm. The linear range was 0.05~2.0 mg/L, the correlation coefficient was 0.9998 and the limit of quantification (S/N=10) was 0.05 mg/kg. The recoveries were 82.1%~102.5% with the relative standard deviations (n=10) of 3.00%~6.25% at the three different spiked levels of 0.05, 0.1 and 1 mg/kg. The method is easy, fast, accurate and consuming less sample and organic solvents. It can be applied in the determination of chlorfluazuron in foods.

Key words: chlorfluazuron, foods, high performance liquid chromatography (HPLC)