色谱

色谱

• 研究论文 • 上一篇    下一篇

高效液相色谱法分析啤酒花浸膏中的6种酸性成分

蔡小明1, 夏璐2, 孙元社2,3, 李彤2,3, 夏明珠1*   

  1. 1. 南京理工大学工业化学研究所, 江苏 南京 210094; 2. 大连依利特分析仪器有限公司, 辽宁 大连 116023; 3. 辽宁大连依利特分析仪器工程技术研究中心, 辽宁 大连 116023
  • 收稿日期:2011-05-18 修回日期:2011-06-10 出版日期:2011-10-28 发布日期:2011-10-13
  • 通讯作者: 夏明珠,副研究员,研究方向为高效液相色谱固定相的制备与应用研究
  • 基金资助:
    江苏省科技支撑计划社会发展项目(No. BE20100731)、辽宁省科学技术计划项目(No. 201002703)和大连市科学技术基金计划项目(No. 2009J22DW015)

Analysis of six acidic components in hops extracts by high performance liquid chromatography

CAI Xiaoming1, XIA Lu2, SUN Yuanshe2,3, LI Tong2,3, XIA Mingzhu1*   

  1. 1. Industrial Chemistry Institute, Nanjing University of Science and Technology, Nanjing 210094, China; 2. Dalian Elite Analytical Instruments Co., Ltd., Dalian 116023, China; 3. Engineering Research Center of Liaoning Dalian Elite Analytical Instruments, Dalian 116023, China
  • Received:2011-05-18 Revised:2011-06-10 Online:2011-10-28 Published:2011-10-13

PDF

449

被引次数

16

摘要： 建立了啤酒花浸膏中6种酸性成分(合葎草酮、葎草酮、加葎草酮、合蛇麻酮、蛇麻酮、加蛇麻酮)的高效液相色谱分析方法。分别考察了酸的加入、有机相种类及柱温对色谱分离效果的影响。在室温条件下,以Hypersil ODS2柱(250 mm×4.6 mm, 5 μm)为分析柱,以乙腈-0.1%(v/v)磷酸水溶液(pH 2.2)(65:35, v/v)为流动相,在1.0 mL/min流速下等度洗脱,于315 nm波长下检测,啤酒花浸膏中6种酸性成分在等度洗脱下实现了基线分离。收集6种酸性成分,分别通过紫外光谱、红外光谱及质谱对其结构进行表征。结果表明该方法具有稳定、简便的优点,适用于啤酒花浸膏中酸性成分的分析。

关键词: 高效液相色谱, 合葎草酮, 合蛇麻酮, 加葎草酮, 加蛇麻酮, 葎草酮, 啤酒花浸膏, 蛇麻酮

Abstract: A method of high performance liquid chromatography (HPLC) was developed for the separation and determination of six acidic components (cohumulone, humulone, adhumulone, colupulone, lupulone and adlupulone) in hops extracts. The effects of several important factors, such as the addition of acid, the organic solvent of elution solution and the column temperature, were investigated to acquire the optimum conditions. The separation was carried out on a Hypersil ODS2 column (250 mm×4.6 mm, 5 μm). A mixture of acetonitrile-0.1%(v/v) phosphoric acid solution (pH 2.2) (65:35, v/v) was used as the mobile phase at a flow rate of 1.0 mL/min in isocratic elution mode. The column temperature was kept at room temperature, and the detection wavelength was set at 315 nm. The six acidic components reached baseline separation, and were identified by ultraviolet spectroscopy, infrared spectroscopy and mass spectrometry. The results show that this method is suitable for the analysis of acidic components in hops extracts owing to the stable and simple performance.

Key words: adhumulone, cohumulone, colupulone, humulone, adlupulone, high performance liquid chromatography (HPLC), hops extract, lupulone