色谱

色谱 ›› 2012, Vol. 30 ›› Issue (07): 721-727.DOI: 10.3724/SP.J.1123.2012.02033

• 研究论文 • 上一篇    下一篇

离子排斥色谱结合光度检测的水质监测系统用于测定离子型营养物（英文）

Daisuke KOZAKI1*, Nobutake NAKATANI2, Masanobu MORI3,Nobukazu NAKAGOSHI1, Kazuhiko TANAKA1,4   

  1. 1. International Development and Cooperation, Hiroshima University, Higashi-Hiroshima 739-8529, Japan; 2. Faculty of Environmental Systems, Rakuno Gakuen University, Hokkaido 069-8501, Japan; 3. Graduate School of Engineering, Gunma University, Gunma 376-8515, Japan; 4. Department of Chemistry, Tsinghua University, Beijing 100084, China
  • 收稿日期:2012-02-22 修回日期:2012-04-01 出版日期:2012-07-28 发布日期:2012-07-19
  • 通讯作者: Daisuke KOZAKI. Tel: +81-82-424-6905, E-mail: Emerald.Green.2-10@hotmail.co.jp.
  • 基金资助:

    This study was financially supported from TOSOH Corporation and Fellowships Program for Young Scientists of the Japan Society for the Promotion of Science on FY2011.

Development of Water Quality Monitoring System for Sequential Determination of Ionic Nutrients by Ion-exclusion Chromatography with Spectrophotometric Detection on Cation- and Anion-exchange Resin Columns in Parallel Using Water Eluent

Daisuke KOZAKI1*, Nobutake NAKATANI2, Masanobu MORI3,Nobukazu NAKAGOSHI1, Kazuhiko TANAKA1,4   

  1. 1. International Development and Cooperation, Hiroshima University, Higashi-Hiroshima 739-8529, Japan; 2. Faculty of Environmental Systems, Rakuno Gakuen University, Hokkaido 069-8501, Japan; 3. Graduate School of Engineering, Gunma University, Gunma 376-8515, Japan; 4. Department of Chemistry, Tsinghua University, Beijing 100084, China
  • Received:2012-02-22 Revised:2012-04-01 Online:2012-07-28 Published:2012-07-19
  • Supported by:

    This study was financially supported from TOSOH Corporation and Fellowships Program for Young Scientists of the Japan Society for the Promotion of Science on FY2011.

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摘要： A unified ion-exclusion chromatography (IEC) system for monitoring anionic and cationic nutrients like NH+4, NO-2, NO-3, phosphate ion, silicate ion and HCO-3was developed and applied to several environmental waters. The IEC system consisted of four IEC methodologies, including the IEC with ultraviolet (UV) detection at 210 nm for determining NH+4on anion-exchange separation column in OH- form connected with anion-exchange UV-conversion column in I- form in tandem, the IEC with UV-detection at 210 nm for determining simultaneously NO-2 and NO-3 on cation-exchange separation column in H+form, the IEC with UV-detection at 210 nm for determining HCO-3on cation-exchange separation column in H+form connected with anion-exchange UV-conversion column in I- form in tandem, and the IEC with visible-detection based on molybdenum-blue reaction for determining simultaneously silicate and phosphate ions on cation-exchange separation column in H+ form. These IEC systems were combined through three manually-driven 6-port column selection valves to select each separation column to determine selectively the ionic nutrients. Using this sequential water quality monitoring system, the analytical performances such as calibration linearity, reproducibility, detection limit and recovery were also tested under the optimized chromatographic conditions. This novel water quality monitoring system has been applied successfully for the determination of the ionic eutrophication components in sub-urban river waters.

关键词: anionic and cationic nutrients, column selection valve, water eluent, ion-exclusion chromatography (IEC)

Abstract: A unified ion-exclusion chromatography (IEC) system for monitoring anionic and cationic nutrients like NH+4, NO-2, NO-3, phosphate ion, silicate ion and HCO-3was developed and applied to several environmental waters. The IEC system consisted of four IEC methodologies, including the IEC with ultraviolet (UV) detection at 210 nm for determining NH+4on anion-exchange separation column in OH- form connected with anion-exchange UV-conversion column in I- form in tandem, the IEC with UV-detection at 210 nm for determining simultaneously NO-2 and NO-3 on cation-exchange separation column in H+form, the IEC with UV-detection at 210 nm for determining HCO-3on cation-exchange separation column in H+form connected with anion-exchange UV-conversion column in I- form in tandem, and the IEC with visible-detection based on molybdenum-blue reaction for determining simultaneously silicate and phosphate ions on cation-exchange separation column in H+ form. These IEC systems were combined through three manually-driven 6-port column selection valves to select each separation column to determine selectively the ionic nutrients. Using this sequential water quality monitoring system, the analytical performances such as calibration linearity, reproducibility, detection limit and recovery were also tested under the optimized chromatographic conditions. This novel water quality monitoring system has been applied successfully for the determination of the ionic eutrophication components in sub-urban river waters.

Key words: anionic and cationic nutrients, column selection valve, water eluent, ion-exclusion chromatography (IEC)