色谱

色谱 ›› 2013, Vol. 31 ›› Issue (2): 139-142.DOI: 10.3724/SP.J.1123.2012.09029

• 研究论文 • 上一篇    下一篇

固相萃取-高效液相色谱法测定地表水中的苯并三唑和苯并噻唑

王金成1, 熊力2, 张海军1, 王龙星1, 金静1, 陈吉平1*   

  1. 1. 中国科学院大连化学物理研究所, 辽宁 大连 116023; 2. 大连工业大学, 辽宁 大连 116034
  • 收稿日期:2012-09-19 修回日期:2012-10-11 出版日期:2013-02-28 发布日期:2013-02-05
  • 通讯作者: 陈吉平
  • 基金资助:

    国家重点基础研究发展计划(“973”计划)项目(2009CB421602).

Simultaneous determination of benzotriazoles and benzothiazole in surface water by solid phase extraction and high performance liquid chromatography

WANG Jincheng1, XIONG Li2, ZHANG Haijun1, WANG Longxing1, JIN Jing1, CHEN Jiping1*   

  1. 1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; 2. Dalian Polytechnic University, Dalian 116034, China
  • Received:2012-09-19 Revised:2012-10-11 Online:2013-02-28 Published:2013-02-05

PDF

419

被引次数

13

摘要： 建立了固相萃取-高效液相色谱分析地表水中苯并噻唑、苯并三唑、5-甲基-苯并三唑、5-氯-苯并三唑及5,6-二甲基-苯并三唑的方法。地表水样品采用HLB固相萃取柱富集净化后,在甲醇-水(55:45, v/v)流动相中用ZORBAX SB-C18色谱柱(250 mm×4.6 mm, 5 μm)分离,紫外检测,外标法定量。结果表明,5种目标化合物在0.064～80 mg/L范围内均呈良好的线性关系,线性相关系数均大于0.9999,仪器检出限为1.9～3.2 μg/L。空白自来水样中的加标回收率为87.8%～125.6%,相对标准偏差(n=3)为0.4%～9.4%。应用该方法测定了大连自来水及辽河入海口水样,在辽河入海口地表水中检出苯并噻唑、苯并三唑、5-甲基-苯并三唑、5-氯-苯并三唑。

关键词: 苯并噻唑, 苯并三唑, 地表水, 高效液相色谱法, 固相萃取, 衍生物

Abstract: A simple method based on solid phase extraction (SPE) coupled with high performance liquid chromatography (HPLC) was developed for the determination of 1H-benzotriazole (BT), 5-methyl-1H-benzotriazole (5-M-BT), 5,6-dimethyl-1H-benzotriazole (5,6-DM-BT), 5-chloro-1H-benzotriazole (5-Cl-BT) and benzothiazole (BTH) in surface water. The extraction and clean-up of the surface water sample were performed on an HLB solid-phase extraction cartridge. The separation of the five compounds was achieved on a ZORBAX SB-C18 column (250 mm×4.6 mm, 5 μm) with methanol-water (55:45, v/v) as mobile phase and ultraviolet detection. The quantification was achieved by external standard method. The results showed that good linearities were observed in the ranges of 0.064-80 mg/L with the correlation coefficients ≥ 0.9999 and the instrumental limits of detection for the five target compounds were in the range of 1.9-3.2 μg/L. The average recoveries of the five target compounds were 87.8%-125.6% and the relative standard deviations (n=3) were 0.4%-9.4%. This method was applied to the analysis of the surface water samples from the Dalian tap water and the Liaohe River estuary. The results showed that BT, 5-M-BT, 5-Cl-BT and BTH were found in the water from the Liaohe River estuary.

Key words: benzothiazole, benzotriazoles, derivatives, high performance liquid chromatography (HPLC), surface water, solid phase extraction (SPE)

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