混合模式色谱分离材料的研究及其应用进展

doi:10.3724/SP.J.1123.2012.12009

色谱 ›› 2013, Vol. 31 ›› Issue (4): 297-302.DOI: 10.3724/SP.J.1123.2012.12009

• 特别策划：色谱固定相专栏 • 上一篇下一篇

混合模式色谱分离材料的研究及其应用进展

董雪芳,蔡晓明,沈爱金,魏杰,郭志谋^*,梁鑫淼^*

中国科学院大连化学物理研究所, 辽宁大连 116023

收稿日期:2012-12-03 出版日期:2013-04-22 发布日期:2013-04-24
通讯作者: Tel:(0411)84379519,E-mail:guozhimou@dicp.ac.cn(郭志谋);E-mail:liangxm@dicp.ac.cn(梁鑫淼)
基金资助:
国家自然科学基金青年基金项目(21005077).

Development and application of separation materials for mixed-mode chromatography

DONG Xuefang, CAI Xiaoming, SHEN Aijin, WEI Jie, GUO Zhimou^*, LIANG Xinmiao^*

1. CAS Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2012-12-03 Online:2013-04-22 Published:2013-04-24

摘要/Abstract

摘要：

近年来混合模式色谱以其独特的分离特性受到人们越来越多的关注。混合模式色谱的种类主要集中在反相/离子交换混合模式色谱(reversed-phase/ion-exchange mixed-mode chromatography, RPLC/IEX),亲水作用/离子交换混合模式色谱(hydrophilic interaction/ion-exchange mixed-mode chromatography, HILIC/IEX),反相/亲水作用混合模式色谱(reversed-phase/hydrophilic interaction mixed-mode chromatography, RPLC/HILIC)等混合模式。两种或多种机理混合使用,往往在分离选择性和色谱峰形等方面能得到不同于单一模式操作所得到的效果,分离选择性以及色谱峰形等都能得到极大的改善与提高,这使得混合模式色谱渐渐进入研究者们的视野。混合模式色谱的研究多数集中在色谱填料的设计。混合模式色谱填料的应用主要针对生物样品的分离分析。该文综述了近年来混合模式色谱的研究及其应用进展，并展望了混合模式色谱的发展。

关键词: 反相/离子交换混合模式色谱, 反相/亲水作用混合模式色谱, 高效液相色谱, 混合模式色谱, 亲水作用/离子交换混合模式色谱, 色谱填料

Abstract:

Mixed-mode chromatography has received more and more attention due to its unique chromatographic characteristics recently. In this review, a summary of the development and applications of mixed-mode chromatography is presented. According to the types of hydrophilic interaction/ion-exchange mixed-mode chromatography (HILIC/IEX), reversed-phase/hydrophilic interaction mixed-mode chromatography (RPLC/HILIC) and reversed-phase/ion-exchange mixed-mode chromatography (RPLC/IEX), the preparation and applications of each type are introduced. Compared with single mode chromatography, the selectivity as well as the sample loading capacity of the mixed-mode chromatography can be improved. The researches mostly focus on the design of mixed-mode stationary phases and the applications especially to bio-analysis. The mixed-mode chromatography will be a potent approach for the analysis and separation of complex samples, and it will also be an effective and complementary tool in practical separation work.

Key words: chromatographic stationary phases, high performance liquid chromatography (HPLC), hydrophilic interaction/ion-exchange mixed-mode chromatography (HILIC/IEX), mixed-mode chromatography, reversed-phase/hydrophilic interaction mixed-mode chromatography (RPLC/HILIC), reversed-phase/ion-exchange mixed-mode chromatography (RPLC/IEX)

中图分类号:

O658

董雪芳,蔡晓明,沈爱金,魏杰,郭志谋^*,梁鑫淼^*. 混合模式色谱分离材料的研究及其应用进展[J]. 色谱, 2013, 31(4): 297-302.

DONG Xuefang, CAI Xiaoming, SHEN Aijin, WEI Jie, GUO Zhimou^*, LIANG Xinmiao^*. Development and application of separation materials for mixed-mode chromatography[J]. Chinese Journal of Chromatography, 2013, 31(4): 297-302.

参考文献

[1] Rafferty J L, Zhang L, Siepmann J I, et al. Anal Chem, 2007, 79(17): 6551

[2] Qiu H D, Liang X J, Sun M, et al. Anal Bioanal Chem, 2011, 399(10): 3307

[3] Miyabe K. J Sep Sci, 2009, 32(5/6): 757

[4] McCalley D V. J Chromatogr A, 2007, 1171(1/2): 46

[5] Francois I, Sandra K, Sandra P. Anal Chim Acta, 2009, 641(1/2): 14

[6] Alpert A J. J Chromatogr, 1990, 499: 177

[7] Fu Q, Liang T, Zhang X L, et al. Carbohydr Res, 2010, 345(18): 2690

[8] Shen A J, Guo Z M, Cai X M, et al. J Chromatogr A, 2012, 1228: 175

[9] Shen A J, Guo Z M, Yu L, et al. Chem Commun, 2011, 47(15): 4550

[10] Gama M R, Silva R G D, Collins C H, et al. Trac-Trends Anal Chem, 2012, 37: 48

[11] Guo H Y, Liu R H, Yang J J, et al. J Chromatogr A, 2012, 1223: 47

[12] Liu Y J, Du Q, Yang B C, et al. Analyst, 2012, 137(7): 1624

[13] West C, Khater S, Lesellier E. J Chromatogr A, 2012, 1250: 182

[14] Yang J, Dong J B, Ye Z D, et al. Acta Chim Sinica, 2012, 70(16): 1725

[15] Zhang F, Li Y, Guo Z, et al. Talanta, 2011, 85(1): 112

[16] Gaur S. J Chromatogr A, 1996, 733(1/2): 57

[17] Weiss J, Jensen D. Anal Bioanal Chem, 2003, 375(1): 81

[18] Chang S P, Wang F Q, Gong B L. Chin J Chem, 2011, 29(4): 793

[19] Hao J X, Wang F Q, Dai X J, et al. Talanta, 2011, 85(1): 482

[20] Davies N H, Euerby M R, McCalley D V. J Chromatogr A, 2007, 1138(1/2): 65

[21] McLaughlin L W. Chem Rev, 1989, 89(2): 309

[22] Yang Y, Geng X D. J Chromatogr A, 2011, 1218(49): 8813

[23] Geng X D. Chinese Journal of Chromatography (耿信笃. 色谱), 2012, 30(1): 1

[24] Mant C T, Hodges R S. J Sep Sci, 2008, 31(15): 2754

[25] Stevenson P G, Fairchild J N, Guiochon G. J Chromatogr A, 2011, 1218(14): 1822

[26] Wu J Y, Bicker W G, Lindner W G. J Sep Sci, 2008, 31(9): 1492

[27] Lammerhofer M, Nogueira R, Lindner W. Anal Bioanal Chem, 2011, 400(8): 2517

[28] Dong J, Ou J J, Dong X L, et al. J Sep Sci, 2007, 30(17): 2986

[29] Lin H, Ou J J, Zhang Z B, et al. Anal Chem, 2012, 84(6): 2721

[30] Borges E M, Euerby M R, Collins C H. Anal Bioanal Chem, 2012, 402(6): 2043

[31] Alpert A J. Anal Chem, 2008, 80(1): 62

[32] Dong X F, Shen A J, Guo Z M, et al. Carbohydr Res, 2012, 361: 195

[33] Guo Z M, Jin Y, Liang T, et al. J Chromatogr A, 2009, 1216(2): 257

[34] Nogueira R, Lubda D, Leitner A, et al. J Sep Sci, 2006, 29(7): 966

[35] Guo Z M, Wang C R, Liang T, et al. J Chromatogr A, 2010, 1217(27): 4555

[36] Cai X M, Guo Z M, Xue X Y, et al. J Chromatogr A, 2012, 1228: 242

[37] Wei J, Guo Z M, Zhang P J, et al. J Chromatogr A, 2012, 1246: 129

[38] Li X L, Guo Z M, Sheng Q Y, et al. Analyst, 2012, 137(12): 2774

[39] Zhu B Y, Mant C T, Hodges R S. J Chromatogr, 1991, 548(1/2): 13

[40] Zhu B Y, Mant C T, Hodges R S. J Chromatogr, 1992, 594(1/2): 75

[41] Mant C T, Kondejewski L H, Hodges R S. J Chromatogr A, 1998, 816(1): 79

[42] Mant C T, Litowski J R, Hodges R S. J Chromatogr A, 1998, 816(1): 65

[43] Litowski J R, Semchuk P D, Mant C T, et al. J Peptide Res, 1999, 54(1): 1

[44] Jiang W, Fischer G, Girmay Y, et al. J Chromatogr A, 2006, 1127(1/2): 82

[45] Liu X D, Pohl C. J Chromatogr A, 2008, 1191(1/2): 83

[46] Liu X D, Pohl C A. J Sep Sci, 2010, 33(6/7): 779

[47] Liu X D, Pohl C, Woodruff A, et al. J Chromatogr A, 2011, 1218(22): 3407

[48] Liu X D, Pohl C A. J Chromatogr A, 2012, 1232: 190

混合模式色谱分离材料的研究及其应用进展

Development and application of separation materials for mixed-mode chromatography

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