色谱

色谱 ›› 2013, Vol. 31 ›› Issue (4): 348-351.DOI: 10.3724/SP.J.1123.2012.02014

• 特别策划：色谱固定相专栏 • 上一篇    下一篇

利用高度结构对称的共聚试剂制备高比表面积的硼亲和整体柱

刘云春,刘震*   

  1. 南京大学化学化工学院 生命分析化学国家重点实验室, 江苏 南京 210093
  • 收稿日期:2013-02-27 出版日期:2013-04-22 发布日期:2013-04-24
  • 通讯作者: Tel:(025)83685639,E-mail:zhenliu@nju.edu.cn
  • 基金资助:

    科技部国际合作项目(35-15);江苏省自然科学基金滚动项目(KB2011054).

Preparation of boronate affinity monolithic column with high specific surface using highly structurally symmetrical copolymerizing reagents

LIU Yunchun, LIU Zhen*   

  1. State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China
  • Received:2013-02-27 Online:2013-04-22 Published:2013-04-24

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摘要：

硼亲和整体柱是一种能对顺式二羟基化合物进行选择性分离的重要色谱介质。然而,整体柱微结构在制备过程中难以控制。本文以分子结构高度对称的三聚氰胺和三(2,3-环氧基丙基)异氰酸酯(TEPIC)作为共聚试剂,利用“团队硼亲和”原理,采用原位聚合的方法制备了一种新型硼亲和整体柱。“团队硼亲和”的作用使该硼亲和整体柱对顺式二羟基化合物的结合pH降低至中性范围。结构高度对称的共聚试剂有效地提高了整体柱材料的比表面积,使其达80.3 m2/g,显著高于文献中报道的其它硼亲和整体柱。

关键词: 比表面积, 硼亲和, 顺式二羟基化合物, 整体柱, 制备

Abstract:

Boronate affinity monolithic column is an important chromatographic medium for the selective isolation of the cis-diol containing compounds. However, it is difficult to control the micro-structure of the monolithic capillary during its preparation process. In this article, using melamine and tris(2,3-epoxypropyl) isocyanurate (TEPIC), both are highly symmetrical in molecular structure, as the copolymerizing reagents, a boronate affinity monolithic capillary with high specific surface was prepared by in-situ polymerization according to the “teamed boronate affinity” principle. The pH for binding with cis-diol containing compounds of this monolithic capillary was decreased to neutral range due to the teamed bonorate affinity. The specific surface area was dramatically increased due to the use of the highly symmetrical copolymerizing reagents, which was 80.3 m2/g and apparently higher than those for other boronate affinity monolithic capillaries reported in references.

Key words: cis-diol containing compounds, boronate affinity, monolithic column, preparation, specific surface

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