色谱

色谱 ›› 2015, Vol. 33 ›› Issue (4): 428-433.DOI: 10.3724/SP.J.1123.2014.12018

• 技术与应用 • 上一篇    下一篇

环果糖色谱柱分离4组结构类似物

舒杨1, 高铭岑1, 易大为2   

  1. 1. 东北大学生命科学与健康学院, 辽宁 沈阳 110169;
    2. 辽宁省药品检验检测院, 辽宁 沈阳 110023
  • 收稿日期:2014-12-15 修回日期:2015-01-09 出版日期:2015-04-08 发布日期:2015-03-30
  • 通讯作者: 舒杨
  • 基金资助:

    国家自然科学基金项目(21105008).

Separations of four groups of analogues on cyclofructan based columns

SHU Yang1, GAO Mingcen1, YI Dawei2   

  1. 1. College of Life and Health Sciences, Northeastern University, Shenyang 110169, China;
    2. Liaoning Institute for Drug Control, Shenyang 110023, China
  • Received:2014-12-15 Revised:2015-01-09 Online:2015-04-08 Published:2015-03-30

PDF

314

被引次数

4

摘要：

考察了聚苯乙烯键合天然环果糖色谱柱MCI Gel CRS100、硅胶键合天然环果糖色谱柱Frulic N、硅胶键合异丙基氨基甲酸酯衍生环果糖色谱柱Larihc P、硅胶键合R-萘乙基氨基甲酸酯衍生环果糖色谱柱Larihc RN在正相模式下对免疫抑制剂、维生素E、姜黄类化合物、辣椒碱4组结构类似物的分离能力。考察了固定相支撑物、固定相功能基团、流动相组成等条件对色谱分离效率的影响。结果显示MCI Gel CRS100由于其固定相较强的疏水性适用于正相色谱而不适用于亲水作用色谱。衍生化的环果糖色谱柱Larihc P和Larihc RN比天然环果糖色谱柱Frulic N具有更高的选择性。三氟乙酸的加入对环果糖色谱柱在正相色谱中分离能力的影响较小。

关键词: 环果糖, 姜黄类化合物, 辣椒碱, 免疫抑制剂, 手性固定相, 维生素E

Abstract:

The separations of the immunosuppressive reagents, capsaicinoids, vitamin E, curcumins on cyclofructan based stationary phases containing polystyrene supported native cyclofructan (MCI Gel CRS100), silica supported native cyclofructan (Frulic N), silica supported isopropyl carbamate cyclofructan 6 (Larihc P), silica supported R-naphthylethyl carbamate cyclofructan 6 (Larihc RN) in normal phase HPLC mode were studied. From the investigation it showed that the column MCI Gel CRS100 was more suitable for the separation in normal phase than in hydrophilic interaction liquid chromatography. Derivatized cyclofructan stationary phases, Larihc P and Larihc RN, showed better selectivities in comparison with the native cyclofructan stationary phases. Trifluoroacetic acid had less influence on resolution on cyclofructan based stationary phases in normal phase mode.

Key words: capsaincinoids, chiral stationary phase, curcumins, cyclofructan, immunosuppressive reagents, vitamin E

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