色谱

色谱 ›› 2016, Vol. 34 ›› Issue (1): 80-84.DOI: 10.3724/SP.J.1123.2015.10039

• 研究论文 • 上一篇    下一篇

多糖类手性固定相超临界流体色谱法分离手性化合物

李冬艳, 吴锡, 郝芳丽, 杨洋, 陈小明   

  1. 大赛璐药物手性技术(上海)有限公司, 上海 200131
  • 收稿日期:2015-10-26 出版日期:2016-01-08 发布日期:2012-08-01
  • 通讯作者: 陈小明

Separation of enantiomers by supercritical fluid chromatography on polysaccharide derivative- based chiral stationary phases

LI Dongyan, WU Xi, HAO Fangli, YANG Yang, CHEN Xiaoming   

  1. Daicel Chiral Technologies (China) Co. Ltd., Shanghai 200131, China
  • Received:2015-10-26 Online:2016-01-08 Published:2012-08-01

PDF

248

被引次数

6

摘要：

采用超临界流体色谱法(SFC),在多糖固定相Chiralpak IA、IB、IC、ID、IE和IF上成功拆分了11种手性化合物。分离结果表明,这6支手性色谱柱对这些手性化合物具有良好的手性识别互补性,均可以在10 min之内得到良好的分离结果,具有较好的实用性。改性剂甲醇、乙醇和异丙醇对手性化合物的保留时间以及手性选择性均具有良好的调节作用,需要根据不同手性物质在手性柱上的分离情况加以区别,选择使用,并调节改性剂至合适的比例。针对键合型固定相溶剂通用性的特征,特殊改性剂的应用也有助于优化手性分离。

关键词: 超临界流体色谱法, 多糖衍生物, 手性分离, 手性固定相

Abstract:

Eleven kinds of chiral compounds have been well separated within 10 min on polysaccharide derivative-based chiral stationary phases named Chiralpak IA, IB, IC, ID, IE and IF by supercritical fluid chromatography (SFC). The chiral recognition of these chiral compounds has demonstrated good complementary enantioselectivities of the six chiral columns, which were proved to be useful for chiral SFC. Both the elution time and enantioselectivies could be significantly affected by the modifier types and their concentrations, such as methanol, ethanol and isopropanol, which should be optimized during the experiments. In addition, the solvent versatility of the immobilized chiral stationary phase on the optimization of the chiral separation was helpful.

Key words: chiral separation, chiral stationary phase, polysaccharide derivatives, supercritical fluid chromatography (SFC)

中图分类号: