色谱

色谱 ›› 2019, Vol. 37 ›› Issue (4): 449-453.DOI: 10.3724/SP.J.1123.2018.10010

• 研究论文 • 上一篇    下一篇

气相色谱-氮磷检测器法表征家蝇对马拉硫磷的水解代谢

张怡, 史雪岩, 高希武   

  1. 中国农业大学植物保护学院, 北京 100193
  • 收稿日期:2018-10-10 出版日期:2019-04-08 发布日期:2015-02-14
  • 通讯作者: 史雪岩.Tel:(010)62731306,E-mail:shixueyan@cau.edu.cn
  • 基金资助:

    国家重点研发计划项目（2018YFD0200408）.

Characterization of hydrolytic metabolism of malathion with Musca domestica by gas chromatography with nitrogen-phosphorus detector

ZHANG Yi, SHI Xueyan, GAO Xiwu   

  1. College of Plant Protection, China Agricultural University, Beijing 100193, China
  • Received:2018-10-10 Online:2019-04-08 Published:2015-02-14
  • Supported by:

    National Key Research and Development Program of China (No. 2018YFD0200408).

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摘要：

为明确家蝇对马拉硫磷的水解代谢能力及相关代谢抗性，在用常规模式底物α-萘乙酯表征了敏感及抗性家蝇的水解酶活性的基础上，使用气相色谱-氮磷检测器（GC-NPD）法比较了抗性及敏感家蝇水解酶对马拉硫磷的水解代谢能力的差异。结果表明，用2：1（v/v）的乙酸乙酯与正己烷混合物萃取后，GC-NPD可成功测定家蝇酶制备液与马拉硫磷温育后的马拉硫磷含量变化，从而可以表征家蝇酶制备液对马拉硫磷的代谢能力。马拉硫磷抗性家蝇水解酶代谢α-萘乙酯的能力是敏感家蝇的1.39倍，代谢马拉硫磷的能力是敏感家蝇的6.68倍，表明家蝇对马拉硫磷的抗性与其对马拉硫磷代谢能力增强有关。该法可用于家蝇等昆虫对马拉硫磷的代谢能力及相关抗性机制研究。

关键词: 代谢抗性, 家蝇, 马拉硫磷, 气相色谱-氮磷检测器

Abstract:

To examine the hydrolytic metabolism and related resistance of housefly Musca domestica (M. domestica) to malathion, differences in the hydrolytic metabolic abilities between malathion-susceptible and malathion-resistant M. domestica were examined using gas chromatography with nitrogen-phosphorus detector (GC-NPD). After incubation of M. domestica abdomen homogenates with malathion, the malathion residue was quantitatively determined by GC-NPD after extraction with a mixture of ethyl acetate and n-hexane at 2:1 (v/v). The hydrolytic metabolic abilities of M. domestica to malathion were then characterized. By comparing the differences in hydrolytic abilities between malathion-susceptible and malathion-resistant M. domestica, it was found that the hydrolytic abilities of malathion-resistant M. domestica were 6.68-fold than that of malathion-susceptible M. domestica, while the hydrolytic abilities toward α-naphthyl acetate were 1.39-fold. Resistance to malathion is related to the enhanced hydrolytic metabolism of malathion by M. domestica. Further understanding of the metabolic resistance mechanism will assist in developing a management strategy for malathion-resistant M. domestica. This method could be used for studying the metabolic ability and related resistance of M. domestica toward malathion.

Key words: Musca domestica, gas chromatography with nitrogen-phosphorus detector (GC-NPD), malathion, metabolic resistance

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