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基于“工文”交叉的仪器分析实验:毛细管电泳-电容耦合非接触电导法测定壁画地仗层中Na+、K+、Ca2+、Mg2+

马洁1, 滕文媛1, 卞雷1,*, 高娟1, 曲昭玮2, 杨玲娟1, 李志锋1, 文娟1, 蒲巧生2,*   

  1. 1.天水师范大学化学工程与技术学院,甘肃 天水 741001;
    2.兰州大学化学化工学院,甘肃 兰州 730000
  • 收稿日期:2026-01-21
  • 通讯作者: *Tel:0938-8367717,E-mail:bianlei@tsnu.edu.cn(卞雷); Tel:0931-8912589,E-mail:puqs@lzu.edu.cn(蒲巧生).
  • 作者简介:马洁. Tel: 0938-8367717, E-mail: majie@tsnu.edu.cn. 博士,副教授.
  • 基金资助:
    天水师范大学教学改革研究项目(PX-123924,PX-123876);甘肃省教育厅高校教师创新基金项目(2024A-116,2026A-141);天水师范大学引进高层次人才科研启动项目;甘肃省基础研究创新群体项目(项目号24JRRE001).

An instrumental analysis experiment based on engineering-liberal arts integration: determination of Na+, K+, Ca2+, and Mg2+ in mural ground layers by capillary electrophoresis with capacitively coupled contactless conductivity detection

MA Jie1, TENG Wenyuan1, BIAN Lei1,*, GAO Juan1, QU Zhaowei2, YANG Lingjuan1, LI Zhifeng1, WEN Juan1, PU Qiaosheng2,*   

  1. 1. College of Chemical Engineering and Technology, Tianshui Normal University, Tianshui 741001, China;
    2. College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China
  • Received:2026-01-21
  • Supported by:
    Tianshui Normal University Teaching Reform Research Project (Nos. PX-123924, PX-123876); Innovation Fund for Colleges and Universities from Department of Education of Gansu Province (Nos. 2024A-116, 2026A-141); Tianshui Normal University Introduced High-Level Talents Research Startup Project; Gansu Provincial Basic Research Innovation Group Project (No. 24JRRE001).

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摘要： 在新工科建设背景下,本文以文物保护实际问题为载体,设计了一项基于“工文”交叉融合的本科仪器分析实验。实验以壁画盐害为研究对象,基于毛细管电泳-电容耦合非接触电导检测(CE-C4D)技术,建立了同时测定Na+、K+、Ca2+和Mg2+的分析方法,并将其用于麦积山石窟壁画地仗层中4种阳离子的定量分析。实验内容包括溶液配制、电泳分离条件优化、方法学考察以及实际样品检测等。该实验具有分析时间短、样品消耗量少、课时安排灵活等优点,能够满足学生独立完成仪器操作与数据采集的需求。通过将文物保护中的真实问题引入仪器分析实验教学,使学生在掌握CE-C4D分析技术的同时,理解分析化学技术在文化遗产保护中的实际应用价值,实现专业知识与人文素养培养的协同发展。

关键词: 毛细管电泳-电容耦合非接触电导检测, 壁画盐害, 离子检测, 新工科

Abstract: In the context of emerging engineering education, an undergraduate instrumental analysis experiment was designed based on the integration of engineering and liberal arts, using a real prob-lem from cultural heritage conservation as the instructional case. The experiment takes mural salt analysis as the research focus. Using capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D), an analytical method was established for the simultaneous deter-mination of Na+, K+, Ca2+, and Mg2+, and the method was applied to the quantitative analysis of these four common cations in the mural ground layers of the Maiji Mountain Grottoes. The experi-mental procedures include solution preparation, optimization of electrophoretic separation condi-tions, analytical method development and validation, and analysis of real samples. The experiment features a short analysis time, low sample consumption, and flexible class-hour arrangement, ena-bling students to independently perform instrument operation and data acquisition. By introducing real issues from cultural heritage conservation into instrumental analysis teaching, the experiment allows students to master CE-C4D analytical techniques while understanding the practical applica-tions of analytical chemistry in cultural heritage protection, thereby promoting the coordinated cul-tivation of professional knowledge and humanistic literacy.

Key words: capillary electrophoresis-capacitively coupled contactless conductivity detection (CE-C4D), mural salt deterioration, ions determination, emerging engineering education

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