色谱

色谱

• “多维色谱”专栏 • 上一篇    下一篇

生物制药研究中的多维色谱

田晶1,2,路鑫1,杨军1,孔宏伟1,王媛1,赵欣捷1, 唐平1,3, 苑广志1,2,许国旺1   

  1. 1. National Chromatographic R. &A. Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116011, China; 2. Analysis Center,　Dalian Institute of Light Industry, Dalian 116001,China; 3. College of Chemistry, Jilin University, Changchun 130021, China
  • 收稿日期:2004-07-30 修回日期:1900-01-01 出版日期:2005-01-30 发布日期:1989-03-25
  • 通讯作者: 许国旺
  • 基金资助:
    国家重点基础研究发展规划(“973”规划)(2003CB716003)和国家“863”计划(2003AA223061)资助项目.

Multidimensional Separations Used in Pharmaceutical and Biological Fields

TIAN Jing1,2, LU Xin1, YANG Jun1, KONG Hongwei1, WANG Yuan1, ZHAO Xinjie1, Guowang xu   

  1. 1. National Chromatographic R. &A. Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116011, China; 2. Analysis Center,　Dalian Institute of Light Industry, Dalian 116001,China; 3. College of Chemistry, Jilin University, Changchun 130021, China
  • Received:2004-07-30 Revised:1900-01-01 Online:2005-01-30 Published:1989-03-25
  • Contact: Guowang xu

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摘要： 对全二维气相色谱(GC×GC)、全二维液相色谱(HPLC×HPLC)、多维毛细管电泳等多维分离技术在生物制药研究中的应用进行了综述，其中对作者所在研究组在全二维气相色谱应用于中药及固相萃取-液相色谱联用分析系统等方面的工作做了重点介绍。由所综述的生物制药研究得出结论：多维分离方法以其高分辨、快速、自动化等特点已经在生物制药领域显示出它的巨大优势,并将发挥更大的作用。

关键词: 多维分离, 联用技术, 色谱, 生物, 制药, 综述

Abstract: A review of multidimensional separations such as comprehensive two-dimensional gas chromatography (GC×GC), comprehensive two-dimensional high performance liquid chromatography (HPLC×HPLC) and their applications in pharmaceutical and biological fields is presented with 71 references. A single CO2 cryo-jet loop modulator was developed for GC×GC and it can be used to modulate compounds higher than C6 effectively. Comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC×GC/TOF-MS) analyses of traditional Chinese medicine volatile oils such as Pogostemon cablin Benth (Cablin Patchouli), Forsythia suspensa (Thunb.) Vahl and Zedoary were reported also. As an emerging technology, multidimensional separations hold the promise and play an important role in the future pharmaceutical and biological fields.

Key words: chromatography, biological, hyphenation techniques, pharmaceutical, review , multidimensional separations