色谱

色谱

• 研究简报 • 上一篇    下一篇

整体柱高效液相色谱法测定尿中核苷的方法研究

赵欣捷,郑育芳,张普敦,孔宏伟,许国旺   

  1. National Chromatographic R. &A. Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116011, China
  • 收稿日期:2004-02-03 修回日期:1900-01-01 出版日期:2005-01-30 发布日期:1989-03-25
  • 通讯作者: 许国旺
  • 基金资助:

High Performance Liquid Chromatographic Method Development Using Monolithic Column for the

ZHAO Xinjie, ZHENG Yufang, ZHANG Pudun, KONG Hongwei, XU Guowang   

  1. National Chromatographic R. &A. Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116011, China
  • Received:2004-02-03 Revised:1900-01-01 Online:2005-01-30 Published:1989-03-25
  • Contact: Guowang xu

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696

被引次数

11

摘要： 尿中修饰核苷已被研究用作可能的肿瘤标记物,目前最常用的分析方法为反相高效液相色谱法,其不足之处是分析周期太长。为此,建立了应用整体柱的高效液相色谱方法对尿中修饰核苷进行分析,所用缓冲液为25 mmol/L磷酸二氢钾溶液(pH 4.55)和60%(体积分数）的甲醇水溶液,线性梯度洗脱,检测波长为260 nm。12种目标核苷可被完全分离。所建立的方法在分离度、线性、重现性、灵敏度及回收率等指标上与普通反相液相色谱法相近,但分析周期大大缩短,仅为23 min,适合于大量临床样品的分析。

关键词: 高效液相色谱法, 核苷, 尿 , 整体柱

Abstract: Modified nucleosides excreted in urine have been studied as possible bio-markers for malignant tumors. Reversed-phase high performance liquid chromatography (RP-HPLC)is one of the most popular analytical tools. However, its analysis time is longer than desired. The analytical method for urinary nucleosides has been developed using a monolithic column and a linear gradient elution of 25 mmol/L KH2PO4 solution (pH 4.55) and 60% methanol in water with UV detection at 260 nm. Twelve urinary nucleosides were completely separated. The monolithic column presented similar analytical characteristics to reversed-phase column. The time of the analysis is only 23 min, in general much shorter than the methods using a packed RP-HPLC. The method developed is sensitive, reliable and suitable for clinical applications.

Key words: monolithic column, nucleosides；urine , high performance liquid chromatography