色谱

色谱

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制备反相高效液相色谱法用于从杉材中分离制备倍半萜类化合物

陈晓辉1,张晖芬1,毕开顺1,金哲史2,崛池 道郎2   

  1. 1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; 2. Department of Bioresources Science, Faculty of Agriculture, Ehime University, Kochi 783-8502, Japan
  • 收稿日期:2004-02-17 修回日期:1900-01-01 出版日期:2005-01-30 发布日期:1989-03-25
  • 通讯作者: 陈晓辉
  • 基金资助:

Isolation and Preparation of Sesquiterpenols from the Japanese Cedar, Cryptomeria japonica D. Don by Preparative Reversed-Phase High Performance Liquid Chromatography and Its Characterization

CHEN Xiaohui1, ZHANG Huifen1, BI Kaishun1, KIM Chul-Sa2, HORIIKE Michio2   

  1. 1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; 2. Department of Bioresources Science, Faculty of Agriculture, Ehime University, Kochi 783-8502, Japan
  • Received:2004-02-17 Revised:1900-01-01 Online:2005-01-30 Published:1989-03-25

PDF

785

被引次数

16

摘要： 利用制备高效液相色谱法从日本产杉材(Cryptomeria japonica D.Don)中分离制备倍半萜类化合物。在YWC-Pack C18柱上,以异丙醇-甲醇-正己烷-水(体积比为50∶35∶10∶5)为流动相,流速为5 mL/min,制备了2种倍半萜类化合物,经紫外光谱、红外光谱及核磁、质谱分析,确认它们分别为(-)-cubebol和(+)-2,7(14),10-bisa-bolatrien-1-ol-4-one。所制备的两个化合物的纯度分别为98.7%和99.1%。

关键词: 倍半萜类化学成分, 杉材 , 制备液相色谱

Abstract: A method for the isolation of sesquiterpenols from the Japanese Cedar, Cryptomeria japonica D.Don　by preparative reversed-phase high performance liquid chromatography (RP-HPLC) was established. The preparation of the sesquiterpenols was carried out on a preparative liquid chromatograph with a YWC-Pack C18 column, and the mobile phase was isopropanol-methanol-hexane-water (50∶35∶10∶5,v/v) at a flow rate of 5.0mL/min. The two sesquiterpenols, (-)-cubebol and (+)-2,7(14),10-bisabolatrien-1-ol-4-one, were separated by liquid chromatography and identified by spectroscopic analyses (ultraviolet (UV), infrared (IR), nuclear magnetic resonance (NMR), electrospray ionization mass spectrometry (ESI-MS)). The purities of the two sesquiterpenols were 98.7% and 99.1%, respectively.

Key words: chemical constituents of sesquiterpenols , Cryptomeria japonica D.Don , preparative high performance liquid chromatography