色谱

色谱

• 研究简报 • 上一篇    下一篇

多种气相色谱联用技术分析陕西刺五加茎挥发油的化学成分

于万滢1,2,张华1,黄威东2,陈吉平2,梁鑫淼2   

  1. 1. State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China; 2. Advanced Analytical Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, China
  • 收稿日期:2004-03-09 修回日期:1900-01-01 出版日期:2005-03-30 发布日期:1988-12-25
  • 通讯作者: 陈吉平
  • 基金资助:

Analysis of the Volatile Oil from the Stem of Acanthopanax senticosus (Rupr. et Maxim.) Harms with Several Hyphenated Methods of Gas Chromatography

YU Wanying1,2, ZHANG Hua1, HUANG Weidong2, CHEN Jiping2, LIANG Xinmiao2   

  1. 1. State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China; 2. Advanced Analytical Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, China
  • Received:2004-03-09 Revised:1900-01-01 Online:2005-03-30 Published:1988-12-25

PDF

551

摘要： 采用气相色谱/四极杆质谱(GC/qMS)、气相色谱/正交加速飞行时间质谱(GC/oaTOFMS)和气相色谱/傅里叶变换红外光谱(GC/FTIR)联用技术,对一种陕西产刺五加Acanthopanax senticosus (Rupr. et Maxim.) Harms茎挥发油的化学成分进行了分析。基于GC/qMS谱库的检索功能,结合GC/FTIR在结构鉴别上的优势和GC/oaTOFMS对质谱碎片离子精确的质量测定功能,成功地实现了对68个色谱组分的定性分析。与使用单一的联用技术(例如GC/qMS)相比,利用多种色谱联用技术在定性分析上的互补性,可以明显提高对组成复杂的挥发油类样品分析的可靠性。

关键词: 刺五加, 挥发油 , 气相色谱/傅里叶变换红外光谱, 气相色谱/四极杆质谱, 气相色谱/正交加速飞行时间质谱

Abstract: Gas chromatography/quadrupole mass spectrometry(GC/qMS), gas chromatography/Fourier transform infrared spectrometry (GC/FTIR) and gas chromatography/orthogonal acceleration time-of-flight mass spectrometry (GC/oaTOFMS) were applied to analyze the volatile oil from the stem of Acanthopanax senticosus ( Rupr. et Maxim.) Harms. Based on the spectra search function of GC/qMS with the aid of the discriminability of the geometrical isomer by GC/FTIR and the ability to determine the accurate mass charge ratio (m/z) by GC/oaTOFMS, 68 GC eluants were identified successfully. Compared with the results obtained by GC/qMS only, the analytical results obtained by these hyphenated methods of gas chromatography are more reliable.

Key words: Acanthopanax senticosus (Rupr. et Maxim.) Harms, gas chromatography/Fourier transform infrared spectrometry, gas chromatography/orthogonal acceleration time-of-flight mass spectrometry, volatile oil , gas chromatography/quadrupole mass spectrometry