色谱

色谱

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气相色谱保留参数用于刺五加果挥发油中萜烯及萜烯氧化物的分类

薛兴亚,于万滢,陈吉平,黄威东,梁鑫淼   

  1. Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, China
  • 收稿日期:2004-06-10 修回日期:1900-01-01 出版日期:2005-07-30 发布日期:1988-06-25
  • 通讯作者: 梁鑫淼
  • 基金资助:

Classification of Terpenes and Terpene Oxides in Volatile Oil of Fruit of Acanthopanax senticosus (Rupr. Et Maxim) Harms with Gas Chromatographic Retention Parameters

XUE Xingya, YU Wanying, CHEN Jiping, HUANG Weidong, LIANG Xinmiao   

  1. Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, China
  • Received:2004-06-10 Revised:1900-01-01 Online:2005-07-30 Published:1988-06-25

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摘要： 根据刺五加挥发油中24个挥发性成分在5个程序升温条件下的保留时间,采用基于Levenberg-Marquardt法的软件GC_AB计算得到相应成分的气相色谱保留参数(A, B值)。通过对其中的萜烯及萜烯氧化物之间A, B关系的相关性分析,发现相同碳数的单萜烯、倍半萜烯和其氧化物分别具有良好的A-B线性相关性,但单萜烯与倍半萜烯、单萜烯与其氧化物、倍半萜烯与其氧化物的A-B关系不共线。研究表明,在中草药挥发油之类的复杂未知混合物体系的分析中,通过色谱保留参数进行萜烯及萜烯氧化物的分类是一种很有潜力的辅助定性方法。

关键词: 刺五加, 分类, 挥发油 , 气相色谱保留参数, 萜烯, 萜烯氧化物

Abstract: Gas chromatographic (GC) retention parameters (A and B values) for 24 volatile compounds in volatile oil of Acanthopanax senticosus (Rupr. Et Maxim) Harms were obtained from the retention times under five temperature-programming conditions with self-developed GC_AB software based on Levenberg-Marquardt method. The correlation analysis between A and B parameters of terpene and its oxides was carried out. Good linear relationships between A and B parameters were built for seven monoterpenes (C10H16), nine sesquiterpenes (C15H24), three monoterpene oxides (C10HnO) and three sesquiterpene oxides (C15HnOx) at the same carbon number, respectively. But poor A-B linearity was built for the group formed from monoterpenes and monoterpene oxides or that formed from sesquiterpenes and sesquiterpene oxides at the same carbon number. At the same time the A-B relationships for monoterpenes, sesquiterpenes and their oxides are not collinear. Therefore, this discipline is applied in the assistant identification for terpenes with different carbon numbers and in the classification between terpenes and their oxides. It shows that GC retention parameters calculated from retention times under several temperature-programming conditions are useful for the classification of monoterpenes, sesquiterpenes and their oxides in the analysis of volatile oils from traditional Chinese herbs.

Key words: Acanthopanax senticosus (Rupr. Et Maxim) Harms, classification, terpene, terpene oxide, volatile oil , gas chromatographic retention parameters