色谱

色谱

• 研究论文 • 上一篇    下一篇

分子印迹薄层色谱手性固定相的制备及其色谱性能

戎非 李萍 冯小刚 袁春伟 付德刚   

  1. 1.State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China; 2.Bioengineering Department, Beijing University of Aeronautics and Astronautics,  Beijing 100083, China
  • 收稿日期:2005-06-21 修回日期:2005-12-15 出版日期:2006-05-30 发布日期:1987-03-25
  • 通讯作者: 付德刚

Preparation and Evaluation of Molecularly Imprinted Stationary Phase Used for Thin-Layer Chromatography

RONG Fei1, LI Ping2, FENG Xiaogang1, YUAN Chunwei1, FU Degang   

  1. 1.State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China; 2.Bioengineering Department, Beijing University of Aeronautics and Astronautics,  Beijing 100083, China
  • Received:2005-06-21 Revised:2005-12-15 Online:2006-05-30 Published:1987-03-25
  • Contact: FU Degang

PDF

1193

被引次数

58

摘要： 分别以右旋扁桃酸、右旋邻氯扁桃酸和右旋对氯扁桃酸为模板,丙烯酰胺、乙二醇二甲基丙烯酸酯为功能单体和交联剂合成分子印迹聚合物，并以此作为薄层色谱手性固定相。研究了模板分子消旋体在手性固定相上的分离情况,并讨论了展开剂中乙酸含量对分离的影响。在乙腈-5%乙酸展开体系中扁桃酸、邻氯扁桃酸和对氯扁桃酸消旋体得到较好的分离,分离因子分别为1.45,1.62和1.56。该手性固定相对模板分子的结构类似物也具有一定的手性交叉分离能力。讨论了分析物的化学结构对该手性固定相识别性能的影响。该方法为快速、灵敏地对手性物质分析、定性提供了一条简便的途径。

关键词: 扁桃酸 , 薄层色谱, 分子印迹聚合物, 手性固定相

Abstract: Thin-layer chromatography (TLC) using molecularly imprinted polymers (MIPs) as chiral stationary phases (CSPs ) was applied to the determination of enantiomers of mandelic acid and its derivatives. In preparation of MIPs, L-mandelic acid, L-2-chloromandelic acid and L-4-chloromandelic acid were employed as templates; acrylamide (AM) and ethylene glycol dimethacrylate (EGDMA) were used as functional monomer and cross-linker respectively. With the development system of acetonitrile-5%acetic acid, the racemates of templates were completely separated on the CSPs, the chiral separation factor α were 1.45, 1.62 and 1.56, respectively. Furthermore, the CSPs were able to resolve the racemates of template’s derivatives. The relationship between the chemical structure of the analytes and chiral recognition of the CSPs was also investigated. This method provides a rapid, sensitive and convenient way of analyzing and determining the enantiomers of chiral compounds.