色谱

色谱

• 研究论文 • 上一篇    下一篇

微乳液毛细管电动色谱法快速测定消炎利胆片中的穿心莲内酯和脱水穿心莲内酯

赵艳芳 罗兴平 明永飞 张红丽 李秀娟 陈立仁 李永民   

  1. 1.Lanzhou Institute of Chemical Physics, the Chinese Academy of Sciences, Lanzhou 730000, China;
    2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
  • 收稿日期:2005-07-12 修回日期:2006-03-09 出版日期:2006-05-30 发布日期:1987-03-25
  • 通讯作者: 陈立仁

Fast Determination of Andrographolide and Dehydroan-drographolide in Xiaoyanlidan Tablets by Microemulsion Electrokinetic Chromatography

ZHAO Yanfang, LUO Xingping, MING Yongfei, ZHANG Hongli, LI Xiujuan, CHEN Liren, LI Yongmin   

  1. 1.Lanzhou Institute of Chemical Physics, the Chinese Academy of Sciences, Lanzhou 730000, China;
    2.Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
  • Received:2005-07-12 Revised:2006-03-09 Online:2006-05-30 Published:1987-03-25
  • Contact:

    Chen Liren

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摘要： 建立了微乳液毛细管电动色谱同时分析消炎利胆片中穿心莲内酯和脱水穿心莲内酯的方法。考察了缓冲溶液的浓度、pH值、十二烷基硫酸钠(SDS)以及助表面活性剂的含量对分离测定的影响。在由乙酸乙酯-SDS-正丁醇-30 mmol/L硼砂缓冲液(pH 9.5)（质量比为0.5∶0.6∶6.0∶92.9）组成的微乳液体系中,两种内酯在6 min内完成分离。该法简便、快速、选择性好,用于实际样品中穿心莲内酯和脱水穿心莲内酯的分析,获得了满意的结果。

关键词: 穿心莲内酯, 脱水穿心莲内酯, 微乳液毛细管电动色谱, 消炎利胆片

Abstract: A microemulsion electrokinetic chromatography (MEEKC) method for the determination of andrographolide and dehydroandrographolide in Xiaoyanlidan Tablets was established. The MEEKC method involved the use of sodium dodecyl sulfate (SDS) as surfactant, ethyl acetate as organic solvent and 1-butanol as co-surfactant. The effects of several factors such as the acidity and concentration of borate buffer, SDS and 1-butanol contents were investigated. The optimized composition (by mass) of microemulsion system are 0.5% ethyl acetate-0.6% SDS-6.0% 1-butanol-92.9% 30 mmol/L sodium tetraborate buffer (pH 9.5). This system allowed a useful and reproducible separation of the analytes to be achieved in less than 6 min. The proposed method is a simple, fast and sensitive method for the determination of andrographolide and dehydroandrographolide in Xiaoyanlidan Tablets.

Key words: andrographolide, dehydroandrographolide, Xiaoyanlidan Tablets , microemulsion electrokinetic chromatography (MEEKC)