色谱

色谱 ›› 2011, Vol. 29 ›› Issue (03): 269-272.DOI: 10.3724/SP.J.1123.2011.00269

• 技术与应用 • 上一篇    下一篇

咪唑离子液体键合硅胶固定相纯水洗脱分离碱基、酚类和药物化合物

王旭生, 邱洪灯, 刘霞, 蒋生祥*   

  1. 中国科学院兰州化学物理研究所, 甘肃省天然药物重点实验室, 甘肃 兰州 730000
  • 收稿日期:2010-12-02 修回日期:2011-02-06 出版日期:2011-03-28 发布日期:2011-03-28
  • 通讯作者: 蒋生祥,研究员,博士生导师.
  • 基金资助:

    国家自然科学基金项目(项目编号: 20905073, 20975105).

Separation of bases, phenols and pharmaceuticals on ionic liquid-modified silica stationary phase with pure water as mobile phase

WANG Xusheng, QIU Hongdeng, LIU Xia, JIANG Shengxiang*   

  1. Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China
  • Received:2010-12-02 Revised:2011-02-06 Online:2011-03-28 Published:2011-03-28

PDF

646

被引次数

10

摘要： 采用N-甲基咪唑和氯丙基咪唑反应的方法制备得到了离子液体键合硅胶固定相,并利用该固定相中的咪唑环阳离子和被分析物之间存在的多重作用机理如疏水作用、静电吸引和排斥及氢键作用等,以纯水作为流动相,成功地分离了碱基(胞嘧啶、胸腺嘧啶、2-氨基嘧啶和6-氯鸟嘌呤)、酚类化合物(间氨基酚、间苯二酚和间硝基酚)以及3种药物化合物(盐酸吗啉呱、阿昔洛韦和头孢氨苄)。采用没有添加任何有机溶剂和缓冲液的纯水作流动相,既绿色环保,又节约经济,简单方便。对该固定相分离这些化合物的保留机理做了探讨。

关键词: 纯水流动相, 酚类化合物, 改性硅胶；固定相, 碱基化合物, 离子液体, 药物, 液相色谱

Abstract: N-methylimidazolium ionic liquid（IL）-modified silica was prepared with the reaction of 3-chloropropyl modified silica and N-methylimidazole using toluene as solvent. Based on the multiple interactions between N-methylimidazolium IL-modified silica and analytes such as hydrophobic interaction, electrostatic attraction, repulsion interaction, hydrogen-bonding, etc., the bases (cytosine, thymine, 2-aminopyrimidine and 6-chloroguanine), phenols (m-aminophenol, resorcinol and m-nitrophenol) and three pharmaceuticals (moroxydine hydrochloride, acyclovir and cephalexin hydrate) were separated successfully with only pure water as the mobile phase. These chromatographic separations are environmental friendly, economical and convenient, without any organic solvent or buffer additive. The retention mechanism of these samples on the stationary phase was also investigated.

Key words: bases, liquid chromatography, modified silica, pharmaceuticals, phenols, pure water as mobile phase, stationary phase, ionic liquid （IL）