色谱

色谱

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高效液相色谱-荧光检测流速梯度法测定去卵巢小鼠脑内单胺类神经递质

张雷,卢英俊,邓同乐,郑筱祥   

  1. Department of Biomedical Engineering of Zhejiang University, Hangzhou 310027, China
  • 收稿日期:2004-02-04 修回日期:1900-01-01 出版日期:2005-01-30 发布日期:1989-03-25
  • 通讯作者: 张雷
  • 基金资助:

Measurement of Monoamine Transmitters in the Brains of Ovariectomized Mice Using High Performance Liquid Chromatography with Fluorescence Detection and Gradient Flow Rate Elution

ZHANG　Lei, LU Yingjun, DENG Tongle, ZHENG Xiaoxiang   

  1. Department of Biomedical Engineering of Zhejiang University, Hangzhou 310027, China
  • Received:2004-02-04 Revised:1900-01-01 Online:2005-01-30 Published:1989-03-25

PDF

702

被引次数

86

摘要： 建立了同时检测去卵巢(OVX)小鼠脑内的去甲肾上腺素(NE)、多巴胺(DA)、 5-羟色胺(5-HT)3种神经递质的高效液相色谱方法。采用的色谱条件为：C18柱分离,荧光检测器检测,流动相为0.1　mol/L的 KH2PO4缓冲液-甲醇(体积比为9∶1),设置单泵流速梯度洗脱。方法的检测限(S/N=3)均在10 nmol/L以下,绝对回收率均在80％以上,样品日内测定峰面积的相对标准偏差在3.03%以下。该方法具有检测灵敏度高、样品制备简便等优点,对小鼠脑内的3种单胺类递质的检测具有实用价值。

关键词: 5-羟色胺, 多巴胺, 高效液相色谱, 流速梯度, 去甲肾上腺素, 去卵巢小鼠

Abstract: A method for the determination of norepinephrine (NE), dopamine (DA) and 5-hydroxytryptamine (5-HT) in brains of ovariectomized mice was established by using high performance liquid chromatograph with a fluorescence detector, a C18 column and a gradient flow rate delivered by a single pump. The mobile phase was a mixture of 0.1 mol/L KH2PO4 and CH3OH (9/1,v/v). Detection limits of the method were determined to be lower than 10 nmol/L, absolute recoveries were above 80% and the relative standard deviations (RSDs) of intra-day were all within 3.03％．The method has the advantages of high sensitivity and of simple sample preparation. Short analysis time and better separation of samples were also obtained.

Key words: 5-hydroxytryptamine, dopamine, gradient flow rate, norepinephrine, ovariectomized mice , high performance liquid chromatography