反相高效液相色谱法测定枇杷叶中的科罗索酸

色谱

反相高效液相色谱法测定枇杷叶中的科罗索酸

胡长平,陈龙胜,忻旸,蔡群兴

Anhui Analysis and Test Center, Hefei 230031, China

收稿日期:2005-08-02 修回日期:2006-01-09 出版日期:2006-09-30 发布日期:1986-09-25
通讯作者: 陈龙胜

Determination of Corosolic Acid in Eriobotrya japonica Leaves by Reversed-Phase High Performance Liquid Chromatography

HU Changping, CHEN Longsheng, XIN Yang, CAI Qunxing

Anhui Analysis and Test Center, Hefei 230031, China

Received:2005-08-02 Revised:2006-01-09 Online:2006-09-30 Published:1986-09-25
Contact: CHEN Longsheng

摘要/Abstract

摘要： 采用反相高效液相色谱法测定了枇杷叶中科罗索酸的含量。将样品在90%乙醇溶液中于90 ℃下提取3.0 ,提取3次,浓缩溶液后加水沉淀,将沉淀溶解于甲醇溶液后在反相色谱柱上分离,以甲醇-1.0%醋酸水溶液(体积比为88∶12)为流动相,流速0.8 mL/min,在波长215 nm处进行测定。结果表明:安徽黄山产枇杷叶中科罗索酸含量（以质量分数计）为0.36%,科罗索酸平均加标回收率为99%。该方法方便快速,结果准确可靠。

关键词: 反相高效液相色谱法, 科罗索酸, 枇杷叶

Abstract: Corosolic acid is clinically proven to activate cell glucose-transporter “shuttles” and thus helps balance blood glucose levels. A method was developed for the determination of corosolic acid in Eriobotrya japonica leaves by reversed-phase high performance liquid chromatography (RP-HPLC). The peak of corosolic acid in Eriobotrya japonica leaves was qualitatively analyzed by comparing the retention times and mass spectra of corosolic acid standard and the extract on HPLC-mass spectrometry (MS). Eriobotrya japonica leaves were extracted thrice for 3.0 h at 90 ℃ with 90% ethanol. The extract was concentrated and deposited by water to remove the impurity which interfered the determination. The deposit was dissolved by methanol and separated on an ODS column (250 mm×4.6 mm i.d., 5 μm). Methanol-1.0%acetic acid (88∶12, v/v) was used as the mobile phase with a flow rate of 0.8 mL/min. The detection wavelength was 215 nm. The linearity was good within the range of 1.0-6.0 μg (r=0.9999). The corosolic acid content of Eriobotrya japonica leaves from Huangshan was 0.36%. The average recovery of corosolic acid was 99%. The method is simple, rapid, accurate and reliable for the determination of corosolic acid in Eriobotrya japonica leaves.

Key words: corosolic acid, Eriobotrya japonica leaves , reversed-phase high performance liquid chromatography(RP-HPLC)

胡长平,陈龙胜,忻旸,蔡群兴. 反相高效液相色谱法测定枇杷叶中的科罗索酸[J]. 色谱.

HU Changping, CHEN Longsheng, XIN Yang, CAI Qunxing. Determination of Corosolic Acid in Eriobotrya japonica Leaves by Reversed-Phase High Performance Liquid Chromatography[J]. Chinese Journal of Chromatography.

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