色谱

色谱 ›› 2014, Vol. 32 ›› Issue (1): 47-51.DOI: 10.3724/SP.J.1123.2013.08019

• 研究论文 • 上一篇    下一篇

应用C30色谱柱高效液相色谱法同时分析枇杷叶中三萜酸类同分异构体

张勇1, 薛昆鹏2, 周安1, 俞慧敏1, 李崟2, 姚立新2, 赵岳星2, 谢晓梅1   

  1. 1. 安徽中医药大学药学院, 安徽 合肥 230031;
    2. 浙江月旭材料科技有限公司, 浙江 金华 321000
  • 收稿日期:2013-08-13 修回日期:2013-09-10 出版日期:2014-01-08 发布日期:2013-12-26
  • 通讯作者: 张勇
  • 基金资助:

    安徽省教育厅自然科学基金项目(KJ2013A172)；安徽中医学院自然科学基金项目(2012zr023).

Determination of triterpenoic acid isomers in Eriobotryae folium using high performance liquid chromatography on C30 column

ZHANG Yong1, XUE Kunpeng2, ZHOU An1, YU Huimin1, LI Yin2, YAO Lixin2, ZHAO Yuexing2, XIE Xiaomei1   

  1. 1. School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230031, China;
    2. Welch Materials (Zhejiang) Inc., Jinhua 321000, China
  • Received:2013-08-13 Revised:2013-09-10 Online:2014-01-08 Published:2013-12-26

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摘要：

建立了一种快速、简便测定中药枇杷叶中4种三萜酸成分的液相色谱分析方法。枇杷叶经甲醇提取并定容后，采用C30色谱柱进行分离，以乙腈-水(95：5，v/v)为流动相进行洗脱，流速为1.0 mL/min，于210 nm波长下检测。对不同产地枇杷叶中的山楂酸、科罗索酸、齐墩果酸和熊果酸4种同分异构体进行了测定和比较。结果表明，该方法分离度好(分离度(R)≥2.2)，精密度高(RSD≤1.1%)，线性关系良好(r≥0.9992)，重现性良好(RSD≤4.4%)，加标回收率范围为95.4%~101.7%(RSD≤4.8%)，满足定量要求。该方法简便、快速，结果可靠，可作为枇杷叶质量评价的方法。

关键词: C30色谱柱, 高效液相色谱法, 科罗索酸, 枇杷叶, 齐墩果酸, 山楂酸, 同分异构体, 熊果酸

Abstract:

A rapid and easy assay method for four triterpenoic acid isomers in Eriobotryae folium was established. The sample was extracted with methanol, then separated on a C30 column (250 mm×4.6 mm, 5 μm) at 20 ℃ using acetonitrile-water (95:5, v/v) as the mobile phase at a flow rate of 1.0 mL/min. The target compounds were simultaneously detected at 210 nm, and the injection volume was 10 μ L. The validation was carried out and the resolution (R≥2.2), the precision (RSD≤1.1%), the linearity (r≥0.9992), the repeatability (RSD≤4.4%), and the recovery (range from 95.4% to 101.7%, RSD≤4.8%) were acceptable. The method is reliable for the quantification of the four triterpenoic acid isomers by HPLC on C30 column.

Key words: Eriobotryae folium, C30 column, corosolic acid, high performance liquid chromatography (HPLC), isomers, maslinic acid, oleanolic acid, ursolic acid

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