色谱

色谱

• 研究论文 • 上一篇    下一篇

快速分离柱高效液相色谱法测定卷烟主流烟气中的主要羰基化合物

黄云1,2,王裔耿1,2,缪明明2,赵琦华1,杨光宇3   

  1. 1.School of Chemical Science and Technology of Yunnan University, Kunming 650091, China; 2.R. &D. Center of Hongta Tobacco Group Co., Ltd, Yuxi 653100, China; 3.Chemical Laboratory, Yunnan Research Institute of Tobacco Science, Kunming 650106, China
  • 收稿日期:2006-08-04 修回日期:2006-09-25 出版日期:2007-03-30 发布日期:1985-12-25
  • 通讯作者: 赵琦华

Determination of Major Carbonyls in Mainstream Smoke by Rapid Column High Performance Liquid Chromatography

HUANG Yun1,2, WANG Yigeng1,2, MIAO Mingming2, ZHAO Qihua1, YANG Guangyu3   

  1. 1.School of Chemical Science and Technology of Yunnan University, Kunming 650091, China; 2.R. &D. Center of Hongta Tobacco Group Co., Ltd, Yuxi 653100, China; 3.Chemical Laboratory, Yunnan Research Institute of Tobacco Science, Kunming 650106, China
  • Received:2006-08-04 Revised:2006-09-25 Online:2007-03-30 Published:1985-12-25
  • Contact: ZHAO Qihua

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摘要： 选择2,4-二硝基苯肼(DNPH)为羰基化合物的衍生化试剂,建立了快速分离柱高效液相色谱测定卷烟主流烟气中8种羰基化合物的方法。采用经2,4-二硝基苯肼酸性溶液处理过的剑桥滤片捕集烟气,再用含2%（体积分数）吡啶的乙腈溶液进行萃取,以ZORBAX Stable Bound色谱柱(50 mm×4.6 mm,1.8 μm)进行快速分离,最后由二极管阵列检测器于365 nm下进行检测。该方法的回收率为89.1%～99.2%,相对标准偏差（RSD)在6.0%以下。该方法分析时间短,流动相消耗少,且操作简便、重复性好、回收率高。

关键词: 2,4-二硝基苯肼 , 高效液相色谱法, 卷烟, 羰基化合物, 主流烟气

Abstract: The determination of major carbonyl compounds in mainstream cigarette smoke by rapid column high performance liquid chromatography was investigated. The cigarette smoke was collected using a Cambridge filter treated with acidic solution of 2,4-dinitrophenylhydrazine. Formaldehyde, acetaldehyde, acetone, acrolein, propionaldehyde, crotonaldehyde, 2-butanone and butyraldehyde were extracted from the Cambridge filter with 50 mL of 2% pyridine acetonitrile solution. The carbonyl compounds in samples were separated on a ZORBAX Stable Bound rapid column (50 mm×4.6 mm, 1.8 μm) in approximately seven minutes and then determined by high performance liquid chromatography with a diode array detector. The average recoveries  were in the range of 89.1% to 99.2% and the relative standard deviations (RSDs) were generally below 6.0%. The eight carbonyl compounds in the mainstream smoke of five brands of cigarettes were determined using this method. This method is faster, simpler and consumes less solvent. It is suitable for rapid analysis of carbonyl compounds in mainstream cigarette smoke.

Key words: carbonyl compounds, cigarette, dinitrophenylhydrazine (DNPH) , mainstream smoke, high performance liquid chromatography (HPLC)