色谱

色谱 ›› 2018, Vol. 36 ›› Issue (8): 817-821.DOI: 10.3724/SP.J.1123.2018.03026

• 技术与应用 • 上一篇    下一篇

基于荷移反应的高效液相色谱法测定氨基丁醇

邵梅琪1, 陈艳1, 彭青1, 柳鹏福1,2, 吴杰群1,2, 储消和1,2   

  1. 1. 浙江工业大学绿色制药协同创新中心, 浙江 杭州 310014;
    2. 浙江省长三角生物医药产业技术研究园, 浙江 德清 313200
  • 收稿日期:2018-03-26 出版日期:2018-08-08 发布日期:2014-06-28
  • 通讯作者: 储消和,E-mail:chuxhe@163.com

Determination of aminobutanol by high performance liquid chromatography based on charge transfer reaction

SHAO Meiqi1, CHEN Yan1, PENG Qing1, LIU Pengfu1,2, WU Jiequn1,2, CHU Xiaohe1,2   

  1. 1. Zhejiang University of Technology, Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Hangzhou 310014, China;
    2. Zhejiang Yangtze Delta Region Pharmaceutical Technology Research Park, Deqing 313200, China
  • Received:2018-03-26 Online:2018-08-08 Published:2014-06-28

PDF

118

摘要：

建立了一种基于荷移反应的高效液相色谱测定氨基丁醇含量的分析方法。在pH 8.4的硼砂-硼酸缓冲溶液中，氨基丁醇与四氯苯醌于60℃反应60 min，利用高效液相色谱法-紫外检测器进行分析。荷移络合物采用Agilent Extend C18色谱柱（250 mm×4.6 mm，5 μm）分离，以0.001%（体积分数）三乙胺甲醇溶液为流动相进行梯度洗脱，流速为1 mL/min，检测波长为350 nm。该方法对氨基丁醇的定量限为0.01 g/L，线性范围为0.1~0.6 g/L，相关系数（R2）为0.9994；方法的加标回收率为98.3%~103.6%，相对标准偏差（RSD）为0.9%~1.6%。该法简便快捷，适用于氨基丁醇含量的快速检测。

关键词: 氨基丁醇, 高效液相色谱法, 荷移反应, 四氯苯醌

Abstract:

A method was developed for the determination of the content of aminobutanol by high performance liquid chromatography (HPLC) based on charge transfer reaction. Under the condition of a borax-boric acid buffer solution of pH 8.4, aminobutanol and tetra-chloro-benzoquinone reacted at 60℃ for 60 min, and were analyzed by an HPLC-ultraviolet detector. The charge-transfer complex was separated on an Agilent Extend C18 column (250 mm×4.6 mm, 5 μm) with 0.001% (v/v) triethylamine and methanol as the mobile phases for gradient elution at a flow rate of 1 mL/min. The limit of quantification of aminobutanol was 0.01 g/L, the linear range was 0.1-0.6 g/L, and the correlation coefficient (R2) was 0.9994. The spiked recoveries of the method were 98.3%-103.6% with relative standard deviations (RSDs) of 0.9%-1.6%. The method is simple and quick, and suitable for the rapid detection of aminobutanol.

Key words: aminobutanol, charge transfer reaction, high performance liquid chromatography (HPLC), tetra-chloro-benzoquinone (TCBQ)

中图分类号: