色谱

色谱

• 技术与应用 • 上一篇    下一篇

微管液相色谱法同时测定白术中的白术内酯Ⅲ、白术内酯Ⅰ和苍术酮

寿旦,戴诗文,章建民,李洪玉,俞忠明   

  1. Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China
  • 收稿日期:2008-10-27 修回日期:2008-10-27 出版日期:2008-10-27 发布日期:1983-09-25
  • 通讯作者: 寿旦
  • 基金资助:
    国家“十五”攻关课题(2004BA721A21)和浙江省分析测试科技计划项目（2007F70067）.

Simultaneous determination of atractylenolide Ⅲ, atractylenolideⅠ and atractylon in Artactylodis

SHOU Dan, DAI Shiwen, ZHANG Jianmin, LI Hongyu, YU Zhongming   

  1. Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310007, China
  • Received:2008-10-27 Revised:2008-10-27 Online:2008-10-27 Published:1983-09-25
  • Contact: SHOU Dan

PDF

547

被引次数

55

摘要： 采用微管液相色谱法同时测定白术中的白术内酯Ⅲ、白术内酯Ⅰ和苍术酮。采用美国Micro-tech Ultra plusⅡTM微管柱通用型二元梯度高效液相色谱仪和Microsil C18微管色谱柱(150 mm×1.0 mm),以甲醇-乙腈-水(A相体积比为90∶5∶5;B相体积比为55∶5∶40)为流动相,梯度洗脱,流速50 μL/min,在220 nm波长下检测。在上述色谱条件下,3种组分分离良好，在相应的进样质量范围内具有良好的线性关系。白术内酯Ⅲ、Ⅰ和苍术酮的加标回收率分别为96.86%,97.13%和98.06%;相对标准偏差分别为1.63%,1.31%和0.39%。该法简便、快捷,能够用于白术的质量控制。

关键词: 白术 , 白术内酯Ⅰ, 白术内酯Ⅲ, 苍术酮, 微管液相色谱法

Abstract: A microbore liquid chromatography (microbore LC) has been developed for the simultaneous determination of atractylenolide Ⅲ, atractylenolideⅠ and atractylon in Artactylodis macrocephala. In the method, a Microsil C18 column (150 mm×1.0 mm) was used with the mobile phase containing methanol-acetonitrile-water for gradient elution, the flow rate was 50 μL/min, and the detection wavelength was set at 220 nm. Under the optimized separation conditions, every component was separated thoroughly. The relationships between the concentrations and the peak areas of the three components were all linear. The recoveries were 96.86% for atractylenolide Ⅲ, 97.13% for atractylenolideⅠand 98.06% for atractylon, the relative standard deviations (RSD) were 1.63%, 1.31% and 0.39%, respectively. The method is simple, convenient, and can be used for the quality control of Artactylodis macrocephala.

Key words: Artactylodis macrocephala , atractylenolide Ⅲ, atractylenolideⅠ, atractylon, microbore liquid chromatography (microbore LC)