色谱

色谱

• 研究论文 • 上一篇    下一篇

基于非手性离子液体的毛细管电泳法拆分3种手性药物

夏陈1 ,陈志涛1,2,夏之宁1,2   

  1. 1.College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, China; 2.College of Bioengineering, Chongqing University, Chongqing 400030, China
  • 收稿日期:2008-04-06 修回日期:2008-07-06 出版日期:2008-11-30 发布日期:1983-06-25
  • 通讯作者: 夏之宁
  • 基金资助:
    国家自然科学基金项目(批准号: 20775096).

Separation of enantiomers of three chiral drugs by capillary electrophoresis based on achiral ionic liquid

XIA Chen1, CHEN Zhitao1,2, XIA Zhining1,2   

  1. 1.College of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, China; 2.College of Bioengineering, Chongqing University, Chongqing 400030, China
  • Received:2008-04-06 Revised:2008-07-06 Online:2008-11-30 Published:1983-06-25
  • Contact: XIA Chen

PDF

912

被引次数

65

摘要： 建立了以非手性离子液体1-正丁基-3-甲基咪唑氯(［BMIM］Cl)为手性分离的添加剂、β-环糊精作为手性选择剂的毛细管区带电泳(CZE)分离扑尔敏、氯霉素前体和氧氟沙星3种对映体的方法,并与未添加［BMIM］Cl的CZE分离情况进行了对比研究。发现［BMIM］Cl对手性药物的拆分有协同作用,不仅能够增加对映体的分离度,还能有效地抑制毛细管内壁对样品分子的吸附作用,改善峰形。采用离子液体辅助手性选择剂(尤其是环糊精)的CZE改进方法,为其他毛细管电泳难以分离的手性药物的分离分析提供了新的方法。

关键词: β-环糊精, 非手性离子液体, 毛细管电泳, 手性拆分, 手性药物

Abstract: Using an achiral ionic liquid, 1-butyl-3-methylimidazolium chlorine (［BMIM］Cl), as an additive and beta-cyclodextrin (β-CD) as a chiral selector, the enantiomers of chlorpheniramine, the precursor of chloramphenicol and ofloxacin were separated by capillary zone electrophoresis. This work was directed to the study of the association of ［BMIM］Cl to the chiral selector β-CD and the possible effects of ［BMIM］Cl on chiral separation. Simultaneously, the separation performances were studied when only containing β-CD in the buffer. The results showed that there are synergistic effects of ［BMIM］Cl as an additive for the enantiomeric separations. ［BMIM］Cl can not only remarkably increase the separation selectivity and resolution of the enantiomers, but also effectively restrain the adsorption of the sample molecules and improve the peak shape. ［BMIM］Cl as an additive of chiral separation can provide a new method for the separation of chiral drugs which are hard separable under common electrophoresis conditions.

Key words: beta-cyclodextrin (β-CD), capillary electrophoresis (CE), chiral drugs , chiral separation, achiral ionic liquid