色谱 ›› 2021, Vol. 39 ›› Issue (6): 559-566.DOI: 10.3724/SP.J.1123.2021.03024

• 年度回顾 • 上一篇    下一篇

2020年毛细管电泳技术年度回顾

魏波1, 马遥1, 田文哲1, 赵新颖2,*(), 屈锋1,*()   

  1. 1.北京理工大学生命学院, 北京 100081
    2.北京电子科技职业学院, 北京 100176
  • 收稿日期:2021-03-17 出版日期:2021-06-08 发布日期:2021-04-13
  • 通讯作者: 赵新颖,屈锋
  • 作者简介:E-mail: 361562040@qq.com(赵新颖).
    * Tel:(010)68918015,E-mail: qufengqu@bit.edu.cn(屈锋);
  • 基金资助:
    国家自然科学基金项目(21874010);国家自然科学基金项目(21827810)

Annual review of capillary electrophoresis technology in 2020

WEI Bo1, MA Yao1, TIAN Wenzhe1, ZHAO Xinying2,*(), QU Feng1,*()   

  1. 1. School of Life Science, Beijing Institute of Technology, Beijing 100081, China
    2. Beijing Polytechnic College, Beijing 100176, China
  • Received:2021-03-17 Online:2021-06-08 Published:2021-04-13
  • Contact: ZHAO Xinying,QU Feng
  • Supported by:
    National Natural Science Foundation of China(21874010);National Natural Science Foundation of China(21827810)

摘要:

该文为2020年毛细管电泳(capillary electrophoresis, CE)技术年度回顾。归纳总结了以“capillary electrophoresis-mass spectrometry”或“capillary isoelectric focusing”或“micellar electrokinetic chromatography”或“capillary electrophoresis”为关键词在ISI Web of Science数据库中进行主题检索得到的2020年CE技术相关研究论文222篇,以及中文期刊《分析化学》和《色谱》中CE技术相关的研究论文37篇。对2020年影响因子(IF)≥5.0的Analytical Chemistry, Food Chemistry, Analytica Chimica ActaTalanta等13本期刊的38篇文章报道的科研工作作了逐一介绍;对IF<5.0的期刊中CE技术报道较为集中的Journal of Chromatography AElectrophoresis两本分析化学类期刊发表40篇文章中的代表性内容作了综合介绍;对重要的中文期刊《分析化学》出版的“核酸适配体专刊”和《色谱》出版的2期CE技术专刊所收录的37篇文章中的工作作了总体介绍。总体来说,2020年CE技术发展趋势仍以毛细管电泳-质谱(CE-MS)的新方法和新应用最为突出,主要集中在CE-MS与电化学检测、固相萃取以及多种毛细管电泳模式的联用方面,CE-MS接口相关的报道较前几年有所减少;常规CE技术则以胶束电动毛细管色谱(MEKC)在复杂样本分析、浓缩富集应用为主,尤其在食品和药品等复杂基质样本分析方面的报道较为集中;此外,我国CE相关领域专家学者的科研成果涵盖了CE在生命科学、临床医学、医药研发、环境科学、天然产物、食品分析等领域的应用,代表了国内CE科研应用水平和现状。

关键词: 毛细管电泳, 2020, 年度回顾

Abstract:

This paper reviews the developments in capillary electrophoresis (CE) in 2020. A total of 222 research papers related to CE technology published in 2020 were retrieved from the ISI Web of Science. These papers were selected by using the keywords “capillary electrophoresis-mass spectrometry”, “capillary isoelectric focusing”, “micellar electrokinetic chromatography”, or “capillary electrophoresis” as search conditions. A further 37 research papers related to CE technology were found in Chinese journals: Chinese Journal of Analytical Chemistry and Chinese Journal of Chromatography. The 38 papers published in 13 different journals with an impact factor (IF)≥5.0 are individually discussed. In particular, the journal Analytical Chemistry (IF=6.8) was found to have nine relevant articles, eight of which were related to CE-MS technology and its application, while Food Chemistry (IF=6.3) published five articles with four papers based on micellar electrokinetic chromatography (MEKC) and its combined technology. This demonstrated that MEKC can simultaneously separate negatively charged, positively charged, and neutral particles, in addition to featuring the advantage of combining different detector applications for the analysis of target matter in a complex matrix. In Analytica Chimica Acta (IF=6.0), five articles were collected, which mainly focused on new applications of CE-MS and multimode CE research. In Talanta (IF=5.3), 10 articles were collected, which focused on new methods and applications of CE and CE-MS. The remaining nine articles, i. e., seven experimental articles, one review article, and one introduction of scientific research achievements, were published in various other high-impact journals. At the same time, 184 articles were found in 89 journals with IF<5.0. In particular, the Journal of Chromatography A (IF=4.1) and Electrophoresis (IF=3.1) contained intensive reports for CE: 16 and 24 publications in relevant areas. The representative contents of 40 articles published in two journals were introduced comprehensively in this review, including new materials and new applications of MEKC, as well as new applications of CE-MS and CE. An overview of the work in the 37 articles collected from Chinese journals included “Aptamer Special Issue” published by Chinese Journal of Analytical Chemistry and two CE technical special issues published by Chinese Journal of Chromatography. In the “Aptamer Special Issue”, Qu Feng’s research group from Beijing Institute of Technology reported five works on aptamers based on CE, while the contents of Chinese Journal of Chromatography include new applications of CE and new materials in capillary tubes. In summary, new methods and applications of capillary electrophoresis-mass spectrometry (CE-MS) were the prominent development trend in CE technology in the year 2020, with the main focus being on the combination of CE-MS with electrochemical detection, solid-phase extraction, and various capillary electrophoresis modes. The number of reports related to the CE-MS interface has decreased compared to that in previous years. Conventional CE technology is mainly focused on the applications of MEKC in the analysis and concentration of complex samples, especially for the analysis of complex matrix samples such as food and drugs. In addition, the scientific research achievements of Chinese experts and scholars in CE-related fields cover the application of CE in life sciences, clinical medicine, pharmaceutical research and development, environmental science, natural products, food analysis, etc., representing the level and current status of the application of CE research in China.

Key words: capillary electrophoresis (CE), 2020, annual review

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