色谱

色谱

• 研究论文 • 上一篇    下一篇

五味子提取物高效液相色谱分析方法的优化

代云桃1, 金高娃2, 秦雪梅1, 薛兴亚2, 章飞芳2,3*, 梁鑫淼2,3   

  1. 1.Research Center for Modern Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China; 2.Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian 116023, China; 3.School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China
  • 收稿日期:2009-02-27 修回日期:2009-04-27 出版日期:2009-07-30 发布日期:1982-06-25
  • 通讯作者: 章飞芳

Optimization of high performance liquid chromatographic method for analysis of extract of Fructus schisandrae chinensis

DAI Yuntao1, JIN Gaowa2, QIN Xuemei1, XUE Xingya2, ZHANG Feifang2,3*, LIANG Xinmiao2,3   

  1. 1.Research Center for Modern Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China; 2.Key Lab of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian 116023, China; 3.School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China
  • Received:2009-02-27 Revised:2009-04-27 Online:2009-07-30 Published:1982-06-25
  • Contact: ZHANG Feifang

PDF

589

被引次数

15

摘要： 针对五味子乙醇提取物的复杂体系,借助于复杂样品分析系统软件(CSASS),根据组分在4次简单线性梯度下42个峰的保留时间,快速准确地计算出各组分的液相色谱保留参数a,c值和峰形参数σ,W1/2。借助这些参数,对五味子色谱的分离情况进行高精度仿真预测。在此基础上,应用移动重叠分辨分离图和谱图仿真技术,发展了计算机辅助的五味子提取物的高效液相色谱全局优化方法。在优化条件下,五味子提取物的高效液相色谱分析可在40 min内完成,且常量成分和部分低含量成分都能够得到较好的分离。所建立的方法已成功地用于五味子中化合物保留时间及峰形的预测,并在此基础上对其色谱分离条件进行优化。

关键词: 复杂样品分析系统软件(CSASS), 高效液相色谱法, 五味子 , 优化

Abstract: For the separation of the complex system of alcohol fraction of Fructus schisandrae chinensis, complex sample analysis system software (CSASS) was utilized to optimize the high performance liquid chromatographic (HPLC) conditions. The HPLC retention parameters a and c, and the peak shape parameters σ and W1/2 were obtained accurately and rapidly by analyzing 4 linear gradient elutions. Based on the 4 parameters, the chromatogram in any gradient condition could be simulated and predicted precisely. The HPLC optimization method was developed using moved overlapping separation ranging map and simulated chromatogram technology. The HPLC analysis of the alcohol fraction of Fructus schisandrae chinensis could be finished in 40 min, and good resolution for components with general or low abundance was achieved. This method could be used to predict retention times and peak shapes of components in Fructus schisandrae chinensis. And based on the precise predicted results, the optimized separation conditions can be obtained.

Key words: complex sample analysis system software (CSASS), Fructus schisandrae chinensi , optimization, high performance liquid chromatography (HPLC)