色谱

色谱

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新型整体材料的制备及其在蛋白质组学中的应用

梁玉, 张丽华*, 张玉奎   

  1. 中国科学院分离分析化学重点实验室, 国家色谱研究分析中心, 中国科学院大连化学物理研究所, 辽宁 大连 116023
  • 收稿日期:2011-08-15 修回日期:2011-08-22 出版日期:2011-09-28 发布日期:2011-10-28
  • 通讯作者: 张丽华,研究员,主要从事生物分子的高效分离与表征等相关研究工作. Tel: (0411)84379720
  • 基金资助:

    国家自然科学基金项目(20935004)、科技部基础研究计划项目(2007CB714503, 2007CB914100)和科技部科技支撑计划项目(2008BAK41B02, 2009BAK59B02).

Preparation of monolithic materials and their applications in proteomic analysis

LIANG Yu, ZHANG Lihua*, ZHANG Yukui   

  1. CAS Key Laboratory of Separation Science for Analytical Chemistry, National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
  • Received:2011-08-15 Revised:2011-08-22 Online:2011-09-28 Published:2011-10-28

PDF

697

被引次数

10

摘要： 蛋白质组学是后基因时代生命科学研究的核心内容之一,其中发展高分辨、高灵敏度、高准确性和高通量的分析技术至关重要。整体材料具有制备简单、传质速度快、色谱柱反压低、表面易修饰等优点,因此已被广泛用于蛋白质组学研究中。本文对各种整体材料(包括有机聚合物整体材料、硅胶整体材料、有机-无机杂化整体材料)的制备方法及其在蛋白质组学研究(如蛋白质的酶解、蛋白质和肽段的分离以及在线酶解-分离-鉴定的高通量分析)中的应用进行了系统综述。

关键词: 蛋白质组学, 应用, 整体材料, 制备, 综述

Abstract: Proteomics is one of the core contents of life science in the post-genomic era, among which it is very important to develop the analytical techniques with high resolution, high sensitivity, high accuracy and high throughput. With the advantages of facile preparation, fast mass transfer, low backpressure and easy modification, monolithic materials have been widely used in proteomic analysis. This review summarizes the preparation methods of different kinds of monolithic materials (including organic polymer monoliths, silica-based monoliths, organic-inorganic hybrid silica monoliths) and their applications in proteomic study such as the digestion of proteins, the separation of proteins or peptides, high throughput analysis integrating online digestion, separation and identification.

Key words: preparation, proteomics, review, applications, monolithic materials