色谱

色谱 ›› 2013, Vol. 31 ›› Issue (11): 1093-1101.DOI: 10.3724/SP.J.1123.2013.05008

• 技术与应用 • 上一篇    下一篇

离子色谱-质谱联用法检测食品中的糖醇

周洪斌1, 熊治渝1,2, 李平1, 李静1, 孙莉1, 赵云霞1   

  1. 1. 国家食品添加剂及调味品检测重点实验室, 江苏 镇江 212008;
    2. 江苏大学, 江苏 镇江 212013
  • 收稿日期:2013-05-03 修回日期:2013-07-04 出版日期:2013-11-28 发布日期:2013-12-05
  • 通讯作者: 周洪斌
  • 基金资助:

    国家质量监督检验检疫总局公益性科研项目(2012424012-3).

Determination of alditols in foods by ion chromatography-mass spectrometry

ZHOU Hongbin1, XIONG Zhiyu1,2, LI Ping1, LI Jing1, SUN Li1, ZHAO Yunxia1   

  1. 1. State Key Laboratory of Food Additive and Condiment Testing, Zhenjiang 212008, China;
    2. Jiangsu University, Zhenjiang 212013, China
  • Received:2013-05-03 Revised:2013-07-04 Online:2013-11-28 Published:2013-12-05

PDF

255

被引次数

19

摘要：

建立了离子色谱-质谱(IC-MS)联用方法检测食品中的糖醇。不同食品样品中的糖醇经提取后过SPE柱去除杂质,再经CarboPar MA1糖醇柱分离,在选择离子监测(SIM)模式下进行检测,外标法定量。各目标物在一定范围内线性关系良好,相关系数(R2)均大于0.99。赤藓糖醇、木糖醇、D-山梨糖醇、D-甘露糖醇、乳糖醇、麦芽糖醇的定量限(S/N=10)分别为0.98、1.99、2.24、5.92、13.56、13.21 mg/kg;检出限(S/N=3)分别为0.28、0.59、0.71、1.74、4.14、4.03 mg/kg。6种糖醇的加标回收率为82.5%~108.0%,相对标准偏差(RSD)为1.5%~7.6%。该方法的灵敏度、准确度和精密度均符合相关的技术要求,适用于食品中糖醇含量的检测。

关键词: 离子色谱-质谱联用, 食品, 糖醇

Abstract:

A method for the determination of alditols in foods by ion chromatography-mass spectrometry (IC-MS) has been developed. The samples were extracted and cleaned up with the solid phase extraction (SPE). Then, the ion chromatographic separation was performed on a CarboPar MA1 column. The alditols were determined by MS with the selected ion monitoring (SIM) mode and quantified by the external standard method. The calibration curves showed good linearity in the certain ranges with the correlation coefficients (R2) greater than 0.99. The limits of quantification (S/N=10) of erythritol, xylitol, D-sorbitol, D-mannitol, lactitol, maltitol were 0.98, 1.99, 2.24, 5.92, 13.56, 13.21 mg/kg and the limits of detection (S/N=3) were 0.28, 0.59, 0.71, 1.74, 4.14, 4.03 mg/kg, respectively. The spiked recoveries of the alditols in the foods at different levels were in the range of 82.5%-108.0% with the relative standard deviations (RSDs) of 1.5%-7.6%. The sensitivity, accuracy and precision of the method meet the technical standards of the determination. The method can be applied to the determination of alditols in foods.

Key words: alditol, food, ion chromatography-mass spectrometry (IC-MS)

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