色谱

色谱 ›› 2013, Vol. 31 ›› Issue (11): 1087-1092.DOI: 10.3724/SP.J.1123.2013.05042

• 技术与应用 • 上一篇    下一篇

液相色谱-质谱法研究附子生物碱的煎煮减毒机理

陈平1, 陈益民1, 陈佳2, 童鸿斌2, 许志良2,3   

  1. 1. 浙江省中医院, 浙江 杭州 310006;
    2. 浙江中医药大学, 浙江 杭州 310053;
    3. 中国科学院大连化学物理研究所, 辽宁 大连 116023
  • 收稿日期:2013-05-27 修回日期:2013-08-22 出版日期:2013-11-28 发布日期:2013-12-05
  • 通讯作者: 许志良
  • 基金资助:

    科技部国际科技合作项目(2009DFA41250).

Exploration of toxicity reducing mechanism of aconite alkaloids during decoction process using liquid chromatography-mass spectrometry

CHEN Ping1, CHEN Yimin1, CHEN Jia2, TONG Hongbin2, XU Zhiliang2,3   

  1. 1. Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou 310006, China;
    2. Zhejiang Chinese Medical University, Hangzhou 310053, China;
    3. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
  • Received:2013-05-27 Revised:2013-08-22 Online:2013-11-28 Published:2013-12-05

PDF

257

被引次数

10

摘要：

建立了基于液相色谱-质谱分析的附子中生物碱指纹分析方法,并将其应用于附子煎煮减毒的机理研究。基于质谱特征鉴定了生物碱标准品在加热回流过程中产生的6种降解产物,确定了双酯型生物碱的煎煮条件降解规律。在此基础上研究了附子饮片煎煮不同时间条件下生物碱的指纹变化,结果表明,长时间煎煮可明显降低双酯型生物碱和生物脂碱的浓度,为附子的安全用药提供了科学依据。

关键词: 附子, 高效液相色谱-质谱, 煎煮, 减毒机理, 生物碱

Abstract:

A high performance liquid chromatography-electrospray ionization mass spectrometry method was developed for the determination of aconite alkaloids. It was used to investigate the degradation of alkaloids of Radix Aconiti Lateralis Preparata during decoction. Six alkaline degradation products were identified, and the degradation regularity of diester-diterpenoid alkaloids was confirmed during the test using standards. The dynamic changes of the amount of aconite alkaloids in the decoction of Radix Aconiti Lateralis Preparata were supervised. Along with the increase of decoction time, the concentrations of diester-diterpenoid alkaloids and lipo-alkaloid decreased significantly. The results can provide a scientific basis for the safety use of aconite.

Key words: alkaloids, decoction, high performance liquid chromatography-mass spectrometry (HPLC-MS), Radix Aconiti Lateralis Preparata, toxicity reducing mechanism

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