顶空气相色谱-质谱测定橄榄调和油中橄榄油含量

doi:10.3724/SP.J.1123.2017.02025

色谱 ›› 2017, Vol. 35 ›› Issue (7): 760-765.DOI: 10.3724/SP.J.1123.2017.02025

顶空气相色谱-质谱测定橄榄调和油中橄榄油含量

蒋万枫, 张宁, 张凤艳, 杨钊

青岛市食品药品检验研究院, 山东青岛 266071

收稿日期:2017-02-24 出版日期:2017-07-08 发布日期:2013-10-18
通讯作者: 蒋万枫,Tel:(0532)85716057,E-mail:jwfvip@126.com

Determination of olive oil content in olive blend oil by headspace gas chromatography-mass spectrometry

JIANG Wanfeng, ZHANG Ning, ZHANG Fengyan, YANG Zhao

Qingdao Institute for Food and Drug Control, Qingdao 266071, China

Received:2017-02-24 Online:2017-07-08 Published:2013-10-18

摘要/Abstract

摘要：

建立了测定橄榄调和油中橄榄油含量的顶空气相色谱-质谱分析方法。对样品量、加热温度、加热时间、进样量、进样模式、色谱柱进行了优化。通过化学计量学方法发现了橄榄油的特征化合物。取1.0 g样品放置于20 mL顶空瓶中，在180 ℃加热振摇2700 s，取1.0 mL顶空气体进样，通过HP-88色谱柱分离和质谱检测。结果表明，方法的线性范围为0~100%（橄榄油含量），线性相关系数（r²）大于0.995，检出限为1.26%~2.13%，模拟橄榄调和油中橄榄油含量测定的偏差为-0.65%~1.02%，相对偏差为-1.3%~6.8%，相对标准偏差为1.18%~4.26%（n=6）。该方法不使用任何溶剂，操作简单、快速、环保，灵敏度和准确度高，适用于橄榄调和油中橄榄油含量的测定。

关键词: 调和油, 顶空气相色谱-质谱, 橄榄油

Abstract:

A method for the determination of the content of olive oil in olive blend oil by headspace gas chromatography-mass spectrometry (SH-GC/MS) was established. The amount of the sample, the heating temperature, the heating time, the amount of injection, the injection mode and the chromatographic column were optimized. The characteristic compounds of olive oil were found by chemometric method. A sample of 1.0 g was placed in a 20 mL headspace flask, and heated at 180℃ for 2700 s. Then, 1.0 mL headspace gas was taken into the instrument. An HP-88 chromatographic column was used for the separation and the analysis was performed by GC/MS. The results showed that the linear range was 0-100%(olive oil content). The linear correlation coefficient (r²) was more than 0.995, and the limits of detection were 1.26%-2.13%. The deviations of olive oil contents in the olive blend oil were from -0.65% to 1.02%, with the relative deviations from -1.3% to 6.8% and the relative standard deviations from 1.18% to 4.26% (n=6). The method is simple, rapid, environment friendly, sensitive and accurate. It is suitable for the determination of the content of olive oil in olive blend oil.

Key words: blend oil, headspace gas chromatography-mass spectrometry (SH-GC/MS), olive oil

中图分类号:

O658

蒋万枫, 张宁, 张凤艳, 杨钊. 顶空气相色谱-质谱测定橄榄调和油中橄榄油含量[J]. 色谱, 2017, 35(7): 760-765.

JIANG Wanfeng, ZHANG Ning, ZHANG Fengyan, YANG Zhao. Determination of olive oil content in olive blend oil by headspace gas chromatography-mass spectrometry[J]. Chinese Journal of Chromatography, 2017, 35(7): 760-765.

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顶空气相色谱-质谱测定橄榄调和油中橄榄油含量

Determination of olive oil content in olive blend oil by headspace gas chromatography-mass spectrometry

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