新型咪唑嵌合氨基亲水作用色谱固定相的制备及其色谱性能

doi:10.3724/SP.J.1123.2014.07022

色谱 ›› 2014, Vol. 32 ›› Issue (10): 1079-1083.DOI: 10.3724/SP.J.1123.2014.07022

新型咪唑嵌合氨基亲水作用色谱固定相的制备及其色谱性能

刘士佳, 乔晓强, 杨艳军, 闫宏远

河北大学药学院, 药物化学与分子诊断教育部重点实验室, 河北保定 071002

收稿日期:2014-07-14 出版日期:2014-10-08 发布日期:2014-09-29
通讯作者: 乔晓强，E-mail：xiaoqiao@hbu.edu.cn
基金资助:
国家自然科学基金项目（21205027）；河北大学自然科学研究计划项目（2011-225）.

Novel imidazolium-embedded amine stationary phase: preparation and its performance for hydrophilic interaction chromatography

LIU Shijia, QIAO Xiaoqiang, YANG Yanjun, YAN Hongyuan

Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Pharmaceutical Sciences, Hebei University, Baoding 071002, China

Received:2014-07-14 Online:2014-10-08 Published:2014-09-29

摘要/Abstract

摘要：

发展新型高效的亲水作用色谱分离材料对于极性化合物的分离分析具有重要的意义。本文设计合成了一种新型咪唑嵌合的氨基亲水作用色谱固定相（Sil-IEASP），并分别采用傅里叶变换红外光谱仪、热重分析仪和元素分析仪对该固定相进行了表征，结果表明该固定相制备成功。以核苷和核酸碱基为样品，分别考察了流动相中的水含量、盐浓度和pH对其保留的影响，结果表明所发展的固定相具有良好的亲水作用特性；此外，缓冲盐浓度和pH几乎不影响上述物质的保留。进一步将该固定相应用于分离尿嘧啶、腺嘌呤、胞嘧啶、尿苷和3种位置异构体（邻三联苯、间三联苯和苯并菲），与常用的氨基固定相相比，本文所发展的固定相具有更好的分离效果，有望在亲水作用色谱分离领域发挥潜在的应用价值。

关键词: 极性化合物, 咪唑嵌合固定相, 亲水作用色谱, 位置异构体

Abstract:

The development of the novel and highly efficient hydrophilic interaction chromatography stationary phase is important for the separation of polar compounds. In the present work, a novel imidazolium-embedded amine stationary phase (Sil-IEASP) was designed and prepared for hydrophilic interaction chromatographic separation. The prepared material was respectively characterized by Fourier transform infrared spectrometer, thermogravimetric analyzer and organic element analyzer. The results indicated that the novel stationary phase was successfully prepared with the present used method. The effects of the contents of water, salt concentration and pH on the retention of nucleosides and nucleic acid bases were respectively investigated, and the results indicated that Sil-IEASP is of hydrophilic interaction chromatographic nature. Furthermore, no obvious effect of buffer pH and salt concentration on the retention of these compounds were found. The developed material was further used for the separation of uracil, adenine, cytosine, uridine and positional isomers (o-terphenyl, m-terphenyl and triphenylene). Compared with the commonly used amino stationary phase, improved separation efficiency was achieved with the developed material, indicating the potential merit of the developed stationary phase for highly efficient hydrophilic interaction chromatographic separation. We anticipate that the novel stationary phase is promising for the hydrophilic interaction chromatography of polar compounds in the future research.

Key words: hydrophilic interaction chromatography, imidazolium-embedded stationary phase, polar compounds, positional isomers

中图分类号:

O658

刘士佳, 乔晓强, 杨艳军, 闫宏远. 新型咪唑嵌合氨基亲水作用色谱固定相的制备及其色谱性能[J]. 色谱, 2014, 32(10): 1079-1083.

LIU Shijia, QIAO Xiaoqiang, YANG Yanjun, YAN Hongyuan. Novel imidazolium-embedded amine stationary phase: preparation and its performance for hydrophilic interaction chromatography[J]. Chinese Journal of Chromatography, 2014, 32(10): 1079-1083.

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新型咪唑嵌合氨基亲水作用色谱固定相的制备及其色谱性能

Novel imidazolium-embedded amine stationary phase: preparation and its performance for hydrophilic interaction chromatography

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