亲水作用色谱-电雾式检测用于5种抗病毒药物的定量分析

doi:10.3724/SP.J.1123.2015.03031

色谱 ›› 2015, Vol. 33 ›› Issue (9): 938-942.DOI: 10.3724/SP.J.1123.2015.03031

亲水作用色谱-电雾式检测用于5种抗病毒药物的定量分析

龙珍¹, 金燕¹, 刘晓达¹, 郭志谋², 沈爱金², 胡兴娟³, 吴宁鹏³

1. 赛默飞世尔(中国)科技有限公司, 北京 100080;
2. 中国科学院分离分析化学重点实验室, 中国科学院大连化学物理研究所, 辽宁大连 116023;
3. 河南省兽药饲料监察所, 河南郑州 450008

收稿日期:2015-03-24 出版日期:2015-09-08 发布日期:2015-09-09
通讯作者: 龙珍, 郭志谋

Quantitative analysis of five antiviral drugs by hydrophilic interaction liquid chromatography-charged aerosol detection

LONG Zhen¹, JIN Yan¹, LIU Xiaoda¹, GUO Zhimou², SHEN Aijin², HU Xingjuan³, WU Ningpeng³

1. ThermoFisher Scientific Corporation, Beijing 100080, China;
2. Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China;
3. Henan Institute of Veterinary Drug Control, Zhengzhou 450008, China

Received:2015-03-24 Online:2015-09-08 Published:2015-09-09

摘要/Abstract

摘要：

抗病毒药物具有广泛的应用,对人类和动物的健康有着重要的作用。但抗病毒药物紫外吸收差异大、极性分布广,要实现该类药物的同时分析较为困难。本实验以Click TE-Cys为固定相,结合电雾式检测(CAD),建立了多种抗病毒药物同时分离分析的方法。考察了不同的检测器、色谱模式、色谱柱和流动相对抗病毒药物峰形、分离选择性及检测信号响应的影响。在优化的条件下定量分析了5种抗病毒药物,结果证明该方法具有较好的日内重复性(RSD≤3.06%)和日间重复性(RSD≤5.38%)。同时,该方法还具有较宽的线性范围(0.07~2.28 mg/mL)和较高的灵敏度(LOQ≤0.04 mg/mL),可用于相关药物的定量、定性分析。

关键词: 电雾式检测, 定量分析, 抗病毒药物, 亲水作用色谱

Abstract:

Antiviral drugs are widely used for human and animals. However, the analysis of the mixture of antiviral drugs is a challenge for high performance liquid chromatography, since some of the antiviral drugs have weak UV absorbance and poor retention in reversed phase liquid chromatography. A method of hydrophilic interaction liquid chromatography-charged aerosol detection (HILIC-CAD) was optimized for the qualitative and quantitative analysis of five antiviral drugs. In this study, Click TE-Cys was used as the stationary phase and CAD was used as the detector. Various chromatographic conditions including the kind of detector, chromatographic mode, column and mobile phase composition were investigated. Compared to UV-Vis, more antiviral drugs could be detected by CAD, since it is a universal detector. HILIC mode is an alternative to reversed phase liquid chromatography mode. HILIC provides higher sensitivity and unique selectivity to target compounds. After the optimized parameters were obtained, the developed method was used for the quantitative analysis of the five antiviral drugs. As a result, the current method has good repeatability, a wide linear range (0.07-2.28 mg/mL) and good sensitivity (LOQ≤0.04 mg/mL). The RSDs of intra-day and inter-day peak areas were less than 3.06% and 5.38% respectively. The above results demonstrated that the current method is sensitive, robust and effective for the separation and determination of these five antiviral drugs.

Key words: antiviral drugs, charged aerosol detection (CAD), hydrophilic interaction liquid chromatography (HILIC), quantitative analysis

中图分类号:

O658

龙珍, 金燕, 刘晓达, 郭志谋, 沈爱金, 胡兴娟, 吴宁鹏. 亲水作用色谱-电雾式检测用于5种抗病毒药物的定量分析[J]. 色谱, 2015, 33(9): 938-942.

LONG Zhen, JIN Yan, LIU Xiaoda, GUO Zhimou, SHEN Aijin, HU Xingjuan, WU Ningpeng. Quantitative analysis of five antiviral drugs by hydrophilic interaction liquid chromatography-charged aerosol detection[J]. Chinese Journal of Chromatography, 2015, 33(9): 938-942.

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亲水作用色谱-电雾式检测用于5种抗病毒药物的定量分析

Quantitative analysis of five antiviral drugs by hydrophilic interaction liquid chromatography-charged aerosol detection

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