色谱

色谱 ›› 2016, Vol. 34 ›› Issue (11): 1084-1090.DOI: 10.3724/SP.J.1123.2016.06057

• 研究论文 • 上一篇    下一篇

梯度淋洗-电导抑制离子色谱法同时测定果汁中26种有机酸和阴离子

宋卫得1, 袁晓鹰1, 吕宁2, 陈太法1, 惠希东1, 苏征1, 金伟1, 刘冰1   

  1. 1. 日照出入境检验检疫局, 山东 日照 276826;
    2. 山东出入境检验检疫局, 山东 青岛 266002
  • 收稿日期:2016-06-30 出版日期:2016-11-08 发布日期:2016-11-03
  • 通讯作者: 宋卫得
  • 基金资助:

    山东出入境检验检疫局科研项目（SK201619）.

Simultaneous determination of 26 organic acids and anions in fruit juice by ion chromatography with suppressed conductivity detection using gradient elution

SONG Weide1, YUAN Xiaoying1, LV Ning2, CHEN Taifa1, HUI Xidong1, SU Zheng1, JIN Wei1, LIU Bing1   

  1. 1. Rizhao Entry-Exit Inspection & Quarantine Bureau, Rizhao 276826, China;
    2. Shandong Entry-Exit Inspection & Quarantine Bureau, Qingdao 266002, China
  • Received:2016-06-30 Online:2016-11-08 Published:2016-11-03
  • Supported by:

    Research Project of Shandong Entry-Exit Inspection & Quarantine Bureau (No. SK201619).

PDF

138

被引次数

6

摘要：

通过对色谱柱类型、流速、柱温、pH值、淋洗液浓度等影响因素的研究，建立了多级梯度淋洗-电导抑制离子色谱同时测定果汁中26种有机酸和阴离子的分析方法。结果表明，当流速为1.00 mL/min、柱温为30℃、pH值为5.5~6.8时，26种组分的测定结果更准确。26种组分在0.02~10.0 mg/L范围内具有良好的线性关系（r均大于0.995），检出限（S/N=3）为0.17~52.0 μg/L；在0.20~2.00 mg/L添加水平下的回收率为85.58%~108.86%，相对标准偏差为0.15%~7.65%（n=6）。该方法简便快速、灵敏度好、准确度高，适于果汁中26种组分的痕量分析。

关键词: 果汁, 离子色谱, 梯度淋洗, 抑制电导检测, 阴离子, 有机酸

Abstract:

A method for simultaneous determination of 26 organic acids and anions in fruit juice by ion chromatography (IC) with suppressed conductivity detection using gradient elution was established. The effects of selection of columns, flow rates, column temperatures, pH values of solution, and eluent concentrations were studied, and then the suitable gradient elution conditions were found for the determination. The testing results of the 26 components in fruit juice were accurate under the conditions that the flow rate was 1.00 mL/min, column temperature was 30℃ and pH values of the solution were in the range of 5.5-6.8. As a result, the linear relationships of the 26 components were good in the range of 0.02-10.0 mg/L and the correlation coefficients (r) were greater than 0.995, the limits of detection (S/N=3) of the 26 components were in the range of 0.17-52.0 μg/L. In the added levels of 0.20-2.00 mg/L, the recoveries of the 26 components in fruit juice ranged from 85.58% to 108.86%, and the relative standard deviations (RSDs) were in the range of 0.15%-7.65% (n=6). The method is simple, quick, sensitive and accurate, and it is absolutely suitable for the trace analysis and determination of the 26 organic acids and anions in fruit juice.

Key words: anions, fruit juice, gradient elution, ion chromatography (IC), organic acids, suppressed conductivity detection

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