色谱

色谱 ›› 2017, Vol. 35 ›› Issue (4): 439-444.DOI: 10.3724/SP.J.1123.2016.09037

• 研究论文 • 上一篇    下一篇

微乳液相色谱法同时分离7种水溶性维生素

吴宏星, 郑艾妮, 苏草茵, 余诺君, 李宁   

  1. 广东药科大学, 广东 广州 510006
  • 收稿日期:2016-09-19 出版日期:2017-04-08 发布日期:2013-07-30
  • 通讯作者: 李宁,Tel:(020)39352136,E-mail:13724117338@163.com.
  • 基金资助:

    国家自然科学基金项目（81173525）.

Simultaneous separation of seven water-soluble vitamins with microemulsion liquid chromatography

WU Hongxing, ZHENG Aini, SU Caoyin, YU Nuojun, LI Ning   

  1. Guangdong Pharmaceutical University, Guangzhou 510006, China
  • Received:2016-09-19 Online:2017-04-08 Published:2013-07-30
  • Supported by:

    National Natural Science Foundation of China (No. 81173525).

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摘要：

采用微乳液相色谱法同时分离7种水溶性维生素（VB1、VB2、VB6、VB12、叶酸、烟酰胺和VC）。考察了微乳流动相体系中表面活性剂、油相、助表面活性剂的种类以及流动相的pH值、柱温等对水溶性维生素分离的影响。优化后微乳体系的组成为：十二烷基硫酸钠（SDS）/聚氧乙烯月桂醇醚（Brij35）/正丁醇/乙酸乙酯/水（质量比为2：60：66：8：864）。色谱柱为Agilent TC C18（250 mm×4.6 mm，5 μm），柱温为30℃，检测波长为254 nm，流速为0.5 mL/min。7种水溶性维生素在20 min内达到基线分离。在4~36 mg/L范围内，7种水溶性维生素的质量浓度与峰面积的相关系数均大于0.9991。不同添加水平下，VB1、VB2、VB6、VC和烟酰胺的平均回收率为93.9%~102.9%。该方法可用于食品和药品中的多种水溶性维生素的分离、鉴别及快速测定。

关键词: 分离选择性, 水溶性维生素, 微乳液相色谱法

Abstract:

A novel method was developed for the separation of seven water-soluble vitamins (VB1, VB2, VB6, VB12, VC, folic acid and nicotinamide) with microemulsion liquid chromatography. The impacts of following factors on the separation selectivity were investigated:the type of surfactant, the type of oil phase, the type of co-surfactant, the pH of mobile phase and the column temperature. The optimal conditions for the separation of the seven water-soluble vitamins were as follows:Agilent TC C18 (250 mm×4.6 mm, 5 μm) was used at 30℃; the microemulsion mobile phase was sodium dodecyl sulfate (SDS)/polyoxyethylene lauryl ether (Brij35)/n-butanol/ethyl acetate/water (2:60:66:80:864, mass ratio). Baseline separation of the seven water-soluble vitamins was achieved within 20 min with a flow rate of 0.5 mL/min and the detection wavelength of 254 nm. The calibration curve showed a good linearity with the correlation coefficients above 0.9991 in the linear range from 4 to 36 mg/L. The mean recoveries of VB1, VB2, VB6, VC and nicotinamide were 93.9%-102.9% at different spiked levels. The optimized and validated method can be used for simultaneous determination of water-soluble vitamins in food and pharmaceuticals.

Key words: microemulsion liquid chromatography (MELC), separation selectivity, water-soluble vitamins

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