色谱

色谱 ›› 2018, Vol. 36 ›› Issue (4): 381-387.DOI: 10.3724/SP.J.1123.2017.11014

• 研究论文 • 上一篇    下一篇

基于加压毛细管电色谱-激光诱导荧光联用技术研究离子强度对维生素B2光解反应的影响

肖汉1, Kevin YAN2, 郑怡婷1, 王彦1, 阎超1   

  1. 1. 上海交通大学药学院, 上海 200240;
    2. University of California, Riverside 92521, USA
  • 收稿日期:2017-11-09 出版日期:2018-04-08 发布日期:2014-02-26
  • 通讯作者: 王彦,E-mail:wangyan11@sjtu.edu.cn;阎超,E-mail:chaoyan@sjtu.edu.cn
  • 基金资助:

    上海市科委科研计划项目（16142200300，17142201000）；上海市引进技术的吸收与创新专项（XC-ZXSJ-02-2016-11）；上海市标准化推进专项（17DZ2201500）.

Study on effect of ionic strength on photolysis of vitamin B2 based on pressurized capillary electrochromatography coupled with laser induced fluorescence detection

XIAO Han1, Kevin YAN2, ZHENG Yiting1, WANG Yan1, YAN Chao1   

  1. 1. School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, China;
    2. University of California, Riverside 92521, USA
  • Received:2017-11-09 Online:2018-04-08 Published:2014-02-26
  • Supported by:

    Shanghai Science and Technology Commission Scientific Research Project (Nos. 16142200300, 17142201000); Shanghai Introduction of Technology Absorption and Innovation (No. XC-ZXSJ-02-2016-11); Shanghai Standardization Promotion Project (No. 17DZ2201500).

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摘要：

基于加压毛细管电色谱-激光诱导荧光检测法建立了分析维生素B2及其荧光性光解产物的方法，并用于研究维生素B2在水溶液和磷酸盐缓冲液中的光解反应速率与离子强度之间的关系。发现在C18毛细管色谱柱，流动相为含0.1%（v/v）三氟乙酸的乙腈水溶液，梯度洗脱，激发波长为488 nm，发射波长为520 nm的条件下，维生素B2及多种荧光性光解产物均得到很好的分离和检测，维生素B2的定量限为5×10-8 mol/L。在此基础上研究了维生素B2的光降解反应受光照时间和离子强度等的影响。发现离子强度对维生素B2溶液的光解反应有显著影响，离子强度越大，光解速度越快。并进一步通过动力学计算得到维生素B2在水溶液和磷酸盐缓冲液中光解反应的表观速率常数。该研究为维生素B2的光稳定研究提供了一种高效分离和检测的方法，并为维生素B2的保存及临床使用提供了参考。

关键词: 反应速率常数, 光解反应, 激光诱导荧光检测, 加压毛细管电色谱, 维生素B2

Abstract:

A new method for the separation and analysis of vitamin B2 and its fluorescent photolysis products was established based on pressurized capillary electrochromatography (pCEC) coupled with laser induced fluorescence (LIF) detection.The relationship between the photolysis reaction rate of vitamin B2 and the ionic strength of aqueous solution or phosphate buffered solution was studied.The analysis was carried out by using a C18 capillary column.The mobile phase was acetonitrile-water with 0.1%(v/v) trifluoroacetic acid with gradient elution.The excitation wavelength was 488 nm and the emission wavelength was 520 nm.Vitamin B2 and many fluorescent photolysis products were well separated.Based on the above, the influence of photo degradation rate of vitamin B2 with ionic strength was studied.The apparent rate constant of photolysis was calculated by kinetic calculation.The results showed that the ionic strength had a significant effect on the photolysis of vitamin B2 solution.The photolysis rate of vitamin B2 was positively related to the ionic strength.This study provides a highly efficient method for the stability research of vitamin B2, which may serve as a good reference for the preservation and clinical use of it.

Key words: laser induced fluorescence detection (LIF), photolysis reaction, pressurized capillary electrochromatography (pCEC), reaction rate constant, vitamin B2

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