色谱

色谱 ›› 2011, Vol. 29 ›› Issue (05): 454-457.DOI: 10.3724/SP.J.1123.2011.00454

• 技术与应用 • 上一篇    下一篇

高效液相色谱-串联质谱法测定化妆品中的欧前胡素和异欧前胡素

陈捷1*, 徐娟1, 熊莉华2   

  1. 1. 广东出入境检验检疫局检验检疫技术中心, 广东 广州 510623; 2. 广州市中小学卫生保健所, 广东 广州 510180
  • 收稿日期:2010-12-27 修回日期:2011-03-03 出版日期:2011-05-28 发布日期:2011-05-28
  • 通讯作者: 陈捷,高级工程师. Tel: (020)38290339,
  • 基金资助:
    检验检疫行业标准制定计划(No. 2009B093).

Determination of imperatorin and isoimperatorin in cosmetics by high performance liquid chromatography-tandem mass spectrometry

CHEN Jie1*, XU Juan1, XIONG Lihua2   

  1. 1. Technology Center of Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China; 2. Health Center of Guangzhou Primary and Middle Schools, Guangzhou 510180, China
  • Received:2010-12-27 Revised:2011-03-03 Online:2011-05-28 Published:2011-05-28

PDF

615

被引次数

5

摘要： 建立了化妆品中欧前胡素和异欧前胡素的高效液相色谱-串联质谱(HPLC-MS/MS)测定与确证方法。样品经甲醇提取,以C18柱为分离柱,10 mmol/L乙酸铵/甲醇溶液-5 mmol/L乙酸铵水溶液(80:20, v/v)为流动相分离,采用电喷雾串联四极杆质谱进行检测。欧前胡素和异欧前胡素在0.25～20 μg/L内线性良好(相关系数大于0.999);方法定量限(LOQ)为0.50 mg/kg。在0.50～10.0 mg/kg内,欧前胡素和异欧前胡素的回收率范围分别为82.2%～105%和80.0%～103%,相对标准偏差分别为2.7%～4.9%和1.8%～4.6%。该方法能够满足化妆品中欧前胡素和异欧前胡素检测的需要。

关键词: 高效液相色谱-串联质谱, 化妆品, 欧前胡素, 异欧前胡素

Abstract: A novel method for the simultaneous determination of imperatorin and isoimperatorin in cosmetics by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed. The imperatorin and isoimperatorin were extracted by methanol. The analytes were separated on a C18 column using 10 mmol/L ammonium acetate/methanol-5 mmol/L ammonium acetate aqueous solution (80:20, v/v) as mobile phase, and then detected by HPLC-MS/MS using multiple reaction monitoring (MRM) mode. The good linearities (r＞0.999) were achieved for the target compounds over the range of 0.25~20 μg/L. The limits of quantification (LOQs) were 0.5 mg/kg. The recoveries for the imperatorin and isoimperatorin varied from 82.2% to 105% and 80.0% to 103% with the relative standard deviations ranging from 2.7% to 4.9% and 1.8% to 4.6% respectively in the range of 0.50~10.0 mg/kg. The method can meet the requirements for the simultaneous determination of imperatorin and isoimperatorin in cosmetics.

Key words: imperatorin, cosmetics, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), isoimperatorin