色谱

色谱 ›› 2012, Vol. 30 ›› Issue (05): 501-506.DOI: 10.3724/SP.J.1123.2011.12006

• 研究论文 • 上一篇    下一篇

毛细管区带电泳法分析不同来源血竭中龙血素A和龙血素B

杨雪滢1, 胡旭芳1, 李菲1, 王兴红2, 曹秋娥1*   

  1. 1. 云南大学化学科学与工程学院 教育部自然资源药物化学重点实验室, 云南 昆明 650091; 2. 云南大学微生物研究所, 云南 昆明 650091
  • 收稿日期:2011-12-02 修回日期:2012-03-07 出版日期:2012-05-28 发布日期:2012-06-07
  • 通讯作者: 曹秋娥,博士,教授,主要研究方向为色谱及分离材料. Tel: (0871)5033723, E-mail: qecao@ynu.edu.cn.
  • 基金资助:

    云南省自然科学基金项目(2008CD066)和云南省科技厅普洱市党政一把手工程项目(2010AE005).

Separation and determination of loureirin A and loureirin B in dragon’s blood by capillary zone electrophoresis

YANG Xueying1, HU Xufang1, LI Fei1, WANG Xinghong2, CAO Qiue1*   

  1. 1. School of Chemical Science and Technology, Yunnan University, Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, Kunming 650091, China; 2. Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China
  • Received:2011-12-02 Revised:2012-03-07 Online:2012-05-28 Published:2012-06-07
  • Contact: Qiu-E CAO

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摘要： 在系统优化了电解质溶液的pH、组成、浓度及仪器条件的基础上,建立了一种测定不同来源血竭中龙血素A和龙血素B的毛细管区带电泳(CZE)方法。采用20 kV的分离电压,25 ℃的毛细管柱温,211 nm的检测波长以及5 s的压力(3447 Pa)进样时间,在20 mmol/L的Na2B4O7缓冲溶液(用NaOH调节pH到9.98,含有10%(v/v,下同)乙腈、5.0%乙二醇和1.0%正丁醇)中,龙血素A和龙血素B在15 min内得到了有效分离与检测。方法的线性范围对于龙血素A和龙血素B分别为1.0～100.0 mg/L和0.5～100.0 mg/L。将该方法用于天然血竭及人工诱导血竭中龙血素A和龙血素B的测定,相对标准偏差在0.6%～3.8%之间,加标回收率在95.1%～105.8%之间。方法具有简单、快速、重现性较好和准确度较高的优点,可以用于血竭样品中龙血素A和龙血素B的测定。

关键词: 龙血素A, 龙血素B, 毛细管区带电泳, 血竭

Abstract: A capillary zone electrophoresis method (CZE) for the simultaneous determination of loureirin A and loureirin B was developed based on the optimized conditions of the pH, composition and concentration of the running buffer solution. Loureirin A and loureirin B were separated and determined effectively within 15 min in a running buffer solution of 20 mmol/L Na2B4O7 (pH 9.98 adjusted with NaOH solution) containing 10.0%(v/v) acetonitrile, 5.0%(v/v) ethylene glycol and 1.0%(v/v) butanol, with the applied voltage of 20 kV, capillary temperature of 25 ℃, detection wavelength of 211 nm, and injection of 5 s at 3447 Pa. The linear ranges for the determination of loureirin A and loureirin B were 1.00~100 mg/L and 0.50~100 mg/L, respectively. The determination of loureirin A and loureirin B in dragon’s blood from natural and artificial inoculation was performed by the proposed method. The relative standard deviations for the determination of the two constituents in samples were from 0.6% to 3.8%, and the recoveries ranged between 95.1% and 105.8%. The method is simple, rapid and possesses higher reproducibility and efficiency. It can be used for the determination of loureirin A and loureirin B in dragon’s blood.

Key words: dragon’s blood, loureirin A, loureirin B, capillary zone electrophoresis (CZE)

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