色谱

色谱 ›› 2012, Vol. 30 ›› Issue (11): 1166-1171.DOI: 10.3724/SP.J.1123.2012.06041

• 研究论文 • 上一篇    下一篇

全二维气相色谱-四极杆质谱法检测植物油脂中脂肪酸

郑月明1,2, 冯峰2, 国伟2, 储晓刚2, 潘家荣1,3*, 贾玮2   

  1. 1. 中国农业科学院农产品加工研究所, 北京 100193; 2. 中国检验检疫科学研究院, 北京 100123; 3. 中国计量学院, 浙江 杭州 310018
  • 收稿日期:2012-06-28 修回日期:2012-08-03 出版日期:2012-11-28 发布日期:2012-11-21
  • 通讯作者: 潘家荣,博士,教授,研究方向为食品安全. E-mail: panjr@263.net.
  • 基金资助:

    国家科技支撑计划课题(2011BAK10B04).

Determination of fatty acids in vegetable oils using comprehensive two-dimensional gas chromatography coupled to quadropole mass spectrometry

ZHENG Yueming1,2, FENG Feng2, GUO Wei2, CHU Xiaogang2, PAN Jiarong1,3*, JIA Wei2   

  1. 1. Institute of Agro-Food Product Processing Science and Technology, Chinese Academy of Agricultural Science, Beijing 100193, China; 2. Chinese Academy of Inspection and Quarantine, Beijing 100123, China; 3. China Jiliang University, Hangzhou 310018, China
  • Received:2012-06-28 Revised:2012-08-03 Online:2012-11-28 Published:2012-11-21

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396

被引次数

16

摘要： 建立了植物油脂中31种脂肪酸成分的全二维气相色谱-四极杆质谱(GC×GC-qMS)分析方法。样品经甲酯化衍生后,以DB-1柱(30 m×0.25 mm×0.25 μm)作为一维柱、DB-Wax柱(3.2 m×0.1 mm×0.1 μm)作为二维柱组成柱系统进行分离,在调制周期为3.5 s、四极杆质量扫描范围为m/z 40～350的条件下,植物油脂中31种脂肪酸成分可以在50 min内得到准确和灵敏的检测。将本方法应用于实际样品的分析,灵敏度较传统的气相色谱-质谱法提高了100倍以上,一些植物油中微量的脂肪酸成分也因此被检出。该研究不仅为植物油中脂肪酸成分的分析提供了新的技术手段,同时对于确保食用植物油的质量安全、消除食用植物油的掺假伪劣等均有重要意义。

关键词: 全二维气相色谱-四极杆质谱, 脂肪酸, 植物油

Abstract: Comprehensive two-dimensional gas chromatography with quadropole mass spectrometry (GC×GC-qMS) was applied to the detection of 31 fatty acids in vegetable oils. The sets of columns, modulation period, scan range of quadropole mass spectrometer were optimized. The results demonstrated that the separation was achieved in 50 min with the column set of DB-1 (30 m×0.25 mm×0.25 μm) as the 1st column and DB-Wax (3.2 m×0.1 mm×0.1 μm) as the 2nd column. All fatty acids were accurately and sensitively determined while the modulation period was 3.5 s and the scan range of quadropole MS was m/z 40~350. Most of the fatty acids were identified by NIST library spectra search, the other fatty acid isomers were identified by single standard injection analysis. When applying this method to the real vegetable oil samples, not only the sensitivities were 100 times higher than those obtained with GC-qMS methods, but also some minor fatty acids were identified. This work suggested a new technical approach in analyzing fatty acid components in vegetable oils, which is meaningful to prohibit adulteration and ensuring the quality safety of edible vegetable oils.

Key words: fatty acids, vegetable oils, comprehensive two-dimensional gas chromatography-quadropole mass spectrometry (GC×GC-qMS)

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