色谱

色谱 ›› 2013, Vol. 31 ›› Issue (12): 1201-1205.DOI: 10.3724/SP.J.1123.2013.07027

• 研究论文 • 上一篇    下一篇

液相色谱-同位素比质谱法同时测定葡萄酒中甘油和乙醇的δ13C值

李学民, 贾光群, 曹彦忠, 张进杰, 王蕾, 孙会媛   

  1. 秦皇岛出入境检验检疫局, 河北 秦皇岛 066004
  • 收稿日期:2013-07-17 修回日期:2013-08-29 出版日期:2013-12-28 发布日期:2013-12-05
  • 通讯作者: 李学民
  • 基金资助:

    国家质检总局科技计划项目（2012IK155）

Simultaneous determination of δ13C values of glycerol and ethanol in wine by liquid chromatography coupled with isotope ratio mass spectrometry

LI Xuemin, JIA Guangqun, CAO Yanzhong, ZHANG Jinjie, WANG Lei, SUN Huiyuan   

  1. Qinhuangdao Entry-Exit Inspection and Quarantine Bureau, Qinhuangdao 066004, China
  • Received:2013-07-17 Revised:2013-08-29 Online:2013-12-28 Published:2013-12-05

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摘要：

采用液相色谱-同位素比质谱（LC-IRMS）技术建立了同时测定葡萄酒中甘油和乙醇δ13C值的分析方法。优化了葡萄酒中影响甘油和乙醇色谱分离的条件。方法的精密度和准确度分别为0.15‰~0.26‰和0.11‰~0.28‰。对40个葡萄酒样品进行了测定，甘油和乙醇的δ13C值分别为-26.87‰~-32.96‰、-24.06‰~-28.29‰，两者具有较强的相关性（R=0.82）。该方法不需要复杂的样品预处理，在相同条件下同时测定甘油和乙醇的δ13C值，较传统方法简单、快速。

关键词: δ13C值, 甘油, 葡萄酒, 液相色谱-同位素比质谱, 乙醇

Abstract:

A novel procedure was established for the characterization of δ13C values of glycerol and ethanol in wine by liquid chromatography-isotope ratio mass spectrometry (LC-IRMS). Several parameters influencing the separation of glycerol and ethanol from wine matrix were optimized. The precision and accuracy of the proposed method were 0.15‰ to 0.26‰ and 0.11‰ to 0.28‰, respectively. The results obtained for 40 wine samples displayed that the δ13C value of glycerol ranged from -26.87‰ to -32.96‰ and that of ethanol ranged from -24.06‰ to -28.29‰. Close correlations (R=0.82) were obtained between the δ13C values of glycerol and ethanol. The proposed method didn't need complex sample treatment, and the δ13C values of glycerol and ethanol in wine can be simultaneously determined, thus improving the method in terms of simplicity and speed compared with traditional methods.

Key words: δ13C values, ethanol, glycerol, liquid chromatography-isotope ratio mass spectrometry (LC-IRMS), wine

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