色谱

色谱 ›› 2014, Vol. 32 ›› Issue (6): 658-661.DOI: 10.3724/SP.J.1123.2014.01023

• 技术与应用 • 上一篇    下一篇

气相色谱-三重四极杆串联质谱稳定同位素稀释法测定被动吸烟儿童尿液中的可丁宁

王芸1,4, 黄志强2, 叶英3, 张莹2, 肖水源1   

  1. 1. 中南大学公共卫生学院, 湖南 长沙 410078;
    2. 湖南省检验检疫科学技术研究院, 湖南 长沙 410004;
    3. 岛津企业管理(中国)有限公司, 广东 广州 510000;
    4. 新疆医科大学护理学院, 新疆 乌鲁木齐 830000
  • 收稿日期:2014-01-13 修回日期:2014-03-30 出版日期:2014-06-08 发布日期:2014-05-22
  • 通讯作者: 黄志强，肖水源
  • 基金资助:

    国家自然科学基金项目（81273089）；中南大学博士生创新基金项目（2013zzts076）.

Determination of urinary cotinine of children exposed to passive smoking by stable isotope dilution gas chromatography-triple quadrupole mass spectrometry

WANG Yun1,4, HUANG Zhiqiang2, YE Ying3, ZHANG Ying2, XIAO Shuiyuan1   

  1. 1. School of Public Health, Central South University, Changsha 410078, China;
    2. Hunan Academy of Inspection and Quarantine, Changsha 410004, China;
    3. Shimadzu Enterprise Management (China) Co., Ltd., Guangzhou 510000, China;
    4. School of Nursing, Xinjiang Medical University, Urumqi 830000, China
  • Received:2014-01-13 Revised:2014-03-30 Online:2014-06-08 Published:2014-05-22

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275

被引次数

1

摘要：

建立了被动吸烟儿童尿液中可丁宁的气相色谱-三重四极杆串联质谱（GC-MS/MS）稳定同位素稀释测定方法。尿液经过三氯甲烷提取、净化，采用气相色谱-三重四极杆串联质谱多反应监测（MRM）模式测定，以可丁宁-d3稳定同位素为内标，定量测定和确证被动吸烟儿童尿液中的可丁宁；在0.1~10 μg/L可丁宁质量浓度范围内方法的线性关系良好，相关系数r>0.998；空白尿液中添加可丁宁0.1、1.0和10 μg/L，回收率为79.2%~112.8%，相对标准偏差在2.1%~5.8%之间；方法定量限达到0.1 μg/L。该方法准确、灵敏、快速，适用于家庭被动吸烟儿童尿液中可丁宁的测定。

关键词: 被动吸烟, 儿童, 可丁宁, 尿液, 气相色谱-串联质谱, 同位素稀释法

Abstract:

An analytical method for the determination of urinary cotinine of children exposed to passive smoking was established based on stable isotope dilution by gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS). The samples were extracted and purified with chloroform. The extracts were determined by GC-MS/MS in multiple reaction monitoring (MRM) mode. The cotinine-d3 as an isotope internal standard was applied to quantify and confirm the urinary cotinine of children exposed to passive smoking. The method had a good linearity from 0.1 μg/L to 10 μg/L with the correlation coefficient (r)>0.998. The recoveries of the cotinine in blank urine were from 79.2% to 112.8% at spiked levels of 0.1, 1.0 and 10 μg/L, with relative standard deviations (RSDs) from 2.1% to 5.8%. The limit of quantification (LOQ) of the method was 0.1 μg/L. The developed method is accurate, sensitive, rapid and can be applied to detect urinary cotinine of children exposed to passive smoking at home.

Key words: children, cotinine, gas chromatography-tandem mass spectrometry (GC-MS/MS), isotope dilution, passive smoking, urine

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