色谱

色谱 ›› 2015, Vol. 33 ›› Issue (2): 158-163.DOI: 10.3724/SP.J.1123.2014.10003

• 技术与应用 • 上一篇    下一篇

高效液相色谱法测定一次性卫生用纺织品中荧光增白剂

魏峰1,2,3, 吴松3, 于晞3, 殷祥刚3, 葛明桥2   

  1. 1. 无锡出入境检验检疫局, 江苏 无锡 214101;
    2. 江南大学生态纺织教育部重点实验室, 江苏 无锡 214122;
    3. 江苏出入境检验检疫局纺织工业产品检测中心, 江苏 无锡 214101
  • 收稿日期:2014-10-10 修回日期:2014-11-17 出版日期:2015-02-08 发布日期:2015-01-24
  • 通讯作者: 魏峰
  • 基金资助:

    江苏省博士后科研资助计划项目(1302164C).

Determination of fluorescent whitening agents in disposable sanitary products by high performance liquid chromatography

WEI Feng1,2,3, WU Song3, YU Xi3, YIN Xianggang3, GE Mingqiao2   

  1. 1. Wuxi Entry-Exit Inspection and Quarantine Bureau, Wuxi 214101, China;
    2. Key Laboratory of Eco-Textiles (Jiangnan University), Ministry of Education, Wuxi 214122, China;
    3. Textile Industry Products Testing Center of Jiangsu Entry-Exit Inspection and Quarantine Bureau, Wuxi 214101, China
  • Received:2014-10-10 Revised:2014-11-17 Online:2015-02-08 Published:2015-01-24

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摘要：

建立了同时测定一次性卫生用纺织产品中4种荧光增白剂(FWA 357、FWA 220、FWA 204和FWA 113)的高效液相色谱-二极管阵列检测器(HPLC-DAD)检测方法。样品用20 mL超纯水作提取剂,于80 ℃下振荡提取30 min,用高效液相色谱进行定性、定量分析。采用SB Phenyl色谱柱(250 mm×4.6 mm, 5 μm)分离,以5 mmol/L乙酸铵和乙腈为流动相梯度洗脱,成功地将待测荧光物质分离。4种荧光增白剂在0.025~400 mg/L范围内线性关系良好,线性相关系数(r2)均大于0.9999;检出限(S/N=3)分别为0.1、0.1、0.02、0.02 mg/L;方法定量限(S/N=10)分别为1.0、2.0、5.0、5.0 mg/kg。添加水平为20~800 mg/kg时,回收率为83.7%~101.0%,相对标准偏差(n=6)为2.4%~7.0%。该方法前处理简单,回收率高,适用于一次性卫生用纺织产品中4种荧光增白剂的测定。

关键词: 纺织品, 高效液相色谱, 一次性卫生用品, 荧光增白剂

Abstract:

A method for the determination of four fluorescent whitening agents (FWAs) (FWA 357, FWA 220, FWA 204 and FWA 113) in disposable sanitary products by high performance liquid chromatography with diode array detection (HPLC-DAD) was developed. The sample was extracted with a 20 mL ultrapure water by sonication for 30 min at 80 ℃. The HPLC method was performed on an SB Phenyl column (250 mm×4.6 mm, 5 μm) by gradient elution using 5 mmol/L ammonium acetate and acetonitrile as mobile phases, which can separate the target analytes successfully. The target analytes showed good linearity in the range of 0.025-400 mg/L with the correlation coefficients (r2) greater than 0.9999. The limits of detection (LODs, S/N=3) were 0.02-0.1 mg/L, and the method limits of quantification (MLOQs) were 1.0-5.0 mg/kg for the target analytes. The feasibility of this method was demonstrated by the determination of FWAs in samples with spiked recoveries. The recoveries were in the range between 83.7% and 101.0%, and the relative standard deviations (RSDs) (n=6) ranged from 2.4% to 7.0%. This method is simple, and has high recoveries for the determination of FWAs in disposable sanitary products.

Key words: disposable sanitary products, fluorescent whitening agents (FWAs), high performance liquid chromatography (HPLC), textiles

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